Skip to Content
Merck

Local ZnCl2 accelerates fracture healing.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society (2014-02-28)
Aaron Wey, Catherine Cunningham, Jeremy Hreha, Eric Breitbart, Jessica Cottrell, Joseph Ippolito, Devin Clark, Hsuan-Ni Lin, Joseph Benevenia, J Patrick O'Connor, Sheldon S Lin, David N Paglia
ABSTRACT

This study evaluated the effect of local zinc chloride (ZnCl2 ), an insulin mimetic agent, upon the early and late parameters of fracture healing in rats using a standard femur fracture model. Mechanical testing, radiographic scoring, histomorphometry, qualitative histological scoring, PCNA immunohistochemistry, and local growth factor analysis were performed. Fractures treated with local ZnCl2 possessed significantly increased mechanical properties compared to controls at 4 weeks post fracture. The radiographic scoring analysis showed increased cortical bridging at 4 weeks in the 1.0 (p=0.0015) and 3.0 (p<0.0001) mg/kg ZnCl2 treated groups. Histomorphometry of the fracture callus at day 7 showed 177% increase (p=0.036) in percent cartilage and 133% increase (p=0.002) in percent mineralized tissue with local ZnCl2 treatment compared to controls. Qualitative histological scoring showed a 2.1× higher value at day 7 in the ZnCl2 treated group compared to control (p = 0.004). Cell proliferation and growth factors, VEGF and IGF-I, within fracture calluses treated with local ZnCl2 were increased at day 7. The results suggest local administration of ZnCl2 increases cell proliferation, causing increased growth factor production which yields improved chondrogenesis and endochondral ossification. Ultimately, these events lead to accelerated fracture healing as early as 4 weeks post fracture.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Zinc chloride solution, 1.0 M in diethyl ether
Sigma-Aldrich
Zinc chloride, reagent grade, ≥98%
Sigma-Aldrich
Zinc chloride solution, 0.5 M in THF
Sigma-Aldrich
Zinc chloride, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥98%
Sigma-Aldrich
Zinc chloride, SAJ first grade, ≥95.0%
Sigma-Aldrich
Zinc chloride solution, 1.9 M in 2-methyltetrahydrofuran
Sigma-Aldrich
Zinc chloride, 99.999% trace metals basis
Sigma-Aldrich
Zinc chloride, AnhydroBeads, amorphous, −10 mesh, 99.999% trace metals basis
Sigma-Aldrich
Zinc chloride, AnhydroBeads, amorphous, −10 mesh, 99.99% trace metals basis
Sigma-Aldrich
Zinc chloride, anhydrous, powder, ≥99.995% trace metals basis
Sigma-Aldrich
Zinc chloride, anhydrous, free-flowing, Redi-Dri, ACS reagent, ≥97%
Sigma-Aldrich
Zinc chloride, ACS reagent, ≥97%
Sigma-Aldrich
Zinc chloride, puriss., meets analytical specification of Ph. Eur., BP, USP, 98-100.5%
Sigma-Aldrich
Zinc chloride, JIS special grade, ≥98.0%
Sigma-Aldrich
Zinc chloride 0.1 M solution
Sigma-Aldrich
Zinc chloride, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture