Skip to Content
Merck
  • Sox2 is the faithful marker for pluripotency in pig: evidence from embryonic studies.

Sox2 is the faithful marker for pluripotency in pig: evidence from embryonic studies.

Developmental dynamics : an official publication of the American Association of Anatomists (2015-01-27)
Shichao Liu, Gerelchimeg Bou, Ruizhen Sun, Shimeng Guo, Binghua Xue, Renyue Wei, Austin J Cooney, Zhonghua Liu
ABSTRACT

Mammalian first lineage segregation generates trophectoderm (TE) and pluripotent inner cell mass (ICM), which provides an ideal model for studying the mechanisms of maintenance and loss of pluripotency. In mouse, the transcription factor OCT4 restricts to ICM and plays a key role in TE/ICM specification and pluripotent regulatory networks. However, in pig, OCT4 does not restrict to ICM cells, suggesting a different molecular basis in TE/ICM specification and pluripotent regulatory networks. To explore molecular basis of porcine TE/ICM specification and pluripotent regulatory networks, we examined expression pattern of pluripotency factors, including SOX2, REX1, SALL4, ESG1, NANOG, TBX3, LIN28, KLF2, and KLF5, in porcine blastocysts. We found that SOX2 is a faithful pluripotent marker that anchored to the pluripotent cells including embryonic part cells, ICM cells and newly EPI cells along with developmental progress, whereas OCT4 expressed in almost all the cells at the same time. Consistently, analysis of spatiotemporal distribution of SOX2 and the TE marker CDX2 revealed an exclusive expression pattern in D6 blastocysts, whereas no correlation was observed between OCT4 and CDX2 at the same stage. Our results provide a molecular basis in porcine embryonic patterning and a clue for further studying porcine pluripotent regulatory networks.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-NANOG antibody produced in goat, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Anti-DPPA5 antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution