SHPMLIBR
MISSION® LentiPlex® Complete Mouse Pooled shRNA Library
For Rapid, Convenient Genome-wide shRNA Screens
About This Item
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product line
MISSION®
concentration
≥5x108 VP/ml (via p24 assay)
technique(s)
capture ELISA: 5 x 108 TU/mL using p24
shipped in
dry ice
storage temp.
−70°C
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General description
The MISSION LentiPlex Complete Mouse shRNA Pooled Library is a genome-wide lentiviral pool produced using a proprietary process. The library consists of over 110,000 shRNA constructs from the TRC collection targeting 21,000+ mouse genes. Each library is tested for shRNA representation before product release to ensure robust library coverage. The library is provided in ready-to-use lentiviral format at titers of at least 5 x 108 TU/ml via p24 assay and is pre-divided into fifteen subpools of approximately 5,500-8,000 shRNA constructs each. Amplification and sequencing primers are also provided for downstream target identification.
For more information including sample data and uses, please visit our LentiPlex Technical Detail Page.
Other Notes
Amplification and sequencing primers are also provided for downstream hit identification.
Deconvolution of shRNA Pools
Sigma′s deconvolution service lets you easily identify the genes that impact your pooled shRNA screen.
- Next-generation sequencing of clonesgives a precise number of individual clone occurrence within a pooled shRNA sample
- Comprehensive, reproducible results from pooled shRNA screens
- Statistically robust and information-rich data
Contact your local Sigma sales representative for more information or submit an inquiry to MISSION RNAi
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Legal Information
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.
When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.
When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.
When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.
Protocols
Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.
Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.
Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.
Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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