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Sigma-Aldrich

Anti-GST Tag Antibody, clone DG122-2A7, HRP conjugate

clone DG122-2A7, Upstate®, from mouse

Synonym(s):

70 kDa zeta-associated protein, Syk-related tyrosine kinase, zeta-chain (TCR) associated protein kinase (70 kD), zeta-chain (TCR) associated protein kinase 70kDa, zeta-chain associated protein kinase 70kDa, zeta-chain associated protein kinase, 70kD

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.56

biological source

mouse

Quality Level

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

DG122-2A7, monoclonal

species reactivity

Schistosoma japonicum

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

General description

Immunogen: GST (Glutathione S-transferase) derived from the pGEX expression vector and linked to KLH.

Specificity: Recognizes GST-fusion proteins.
Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The amino acid sequence GST is highly conserved in most organisms, including mammals.

Specificity

Recognizes GST-fusion proteins.

Immunogen

GST (Glutathione S-transferase) derived from the pGEX expression vector and linked to KLH. Clone DG122-2A7.

Application

Anti-GST Tag Antibody, clone DG122-2A7, HRP conjugate detects level of GST Tag & has been published & validated for use in WB.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Quality

Routinely evaluated by western blot Analysis on recombinant GST protein.

Western Blot Analysis:
1:500-1:1000 dilution of this lot detected recombinant GST protein.

Physical form

Protein G Purified
Purified mouse monoclonal IgG in buffer containing (HRP) in 70% storage buffer PBS with 0.05% Kathon® and 30% glycerol. Liquid at -20°C.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Control
Cells expressing the GST-tag.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

Kathon is a registered trademark of Rohm and Haas Co.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictograms

Exclamation markEnvironment

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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