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04-262

Sigma-Aldrich

Anti-Ubiquitinylated proteins Antibody, clone FK1

clone FK1, Upstate®, from mouse

Synonym(s):

Anti-Ubiquitin Antibody

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

FK1, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgM

shipped in

wet ice

target post-translational modification

unmodified

General description

Ubiquitin is a polypeptide of 76 amino acid residues, being a very highly conserved and widely distributed protein in all eukaryotic cells. It has been proposed that ubiquitin plays important roles in various biological processes such as the regulation of cell cycle, DNA replication, DNA repair, stress response, apoptosis, signal transduction, and the biogenesis of ribosome, nucleosome, peroxisome and myofibrils. In these processes, ubiquitin functions as both a signal for protein degradation and as a chaperone promoting the formation of organelles.

Specificity

Recognizes only poly-ubiquitinylated proteins and not mono-ubiquitinylated proteins or free ubiquitin.

Immunogen

Poly-ubiquitinylated-lysozyme.

Application

Anti-Ubiquitinylated proteins Antibody, clone FK1 is an antibody against Ubiquitinylated proteins for use in WB, IH.
Research Category
Protein Trafficking
Research Sub Category
Ubiquitin & Ubiquitin Metabolism

Physical form

100 µL of concentration 1mg/mL purified immunoglobulin in PBS containing 0.1% sodium azide. Dilute to working strength with phosphate buffered saline pH 7.2-7.4.
Format: Purified

Storage and Stability

Stable for 1 year at 2-8°C from date of shipment for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kaisa Haglund et al.
Nature cell biology, 5(5), 461-466 (2003-04-30)
Many cellular proteins are post-translationally modified by the addition of a single ubiquitin or a polyubiquitin chain. Among these are receptor tyrosine kinases (RTKs), which undergo ligand-dependent ubiquitination. The ubiquitination of RTKs has become recognized as an important signal for
Haopeng Wang et al.
The EMBO journal, 29(7), 1285-1298 (2010-02-13)
Expression of the T-cell receptor (TCR):CD3 complex is tightly regulated during T-cell development. The mechanism and physiological role of this regulation are unclear. Here, we show that the TCR:CD3 complex is constitutively ubiquitylated in immature double positive (DP) thymocytes, but
Hugues Lelouard et al.
Nature, 417(6885), 177-182 (2002-05-10)
Dendritic cells (DCs) are antigen-presenting cells with the unique capacity to initiate primary immune responses. Dendritic cells have a remarkable pattern of differentiation (maturation) that exhibits highly specific mechanisms to control antigen presentation restricted by major histocompatibility complex (MHC). MHC
Javier H Jara et al.
Cerebral cortex (New York, N.Y. : 1991), 25(11), 4259-4272 (2015-01-18)
Corticospinal motor neurons (CSMN) receive, integrate, and relay cerebral cortex's input toward spinal targets to initiate and modulate voluntary movement. CSMN degeneration is central for numerous motor neuron disorders and neurodegenerative diseases. Previously, 5 patients with mutations in the ubiquitin
z-Leucinyl-leucinyl-norleucinal induces apoptosis of human glioblastoma tumor-initiating cells by proteasome inhibition and mitotic arrest response.
Monticone M, Biollo E, Fabiano A, Fabbi M, Daga A, Romeo F, Maffei M, Melotti A et al.
Molecular Cancer Research null

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