The molar concentration is 9.93E-09 M. This can be calculated using the weight concentration of 6.07E-02 and the molar mass of 6.11E+06.
741957
Gold nanoparticles
10 nm diameter, OD 1, stabilized suspension in citrate buffer
Synonym(s):
Au NP, Gold Colloid
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About This Item
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Quality Level
form
nanoparticles
suspension
contains
Proprietary Surfactant as stabilizer
concentration
~6.0E+12 particles/mL
OD
1
diameter
10 nm
λmax
515-519 nm
PDI
<0.2
storage temp.
2-8°C
SMILES string
[Au]
InChI
1S/Au
InChI key
PCHJSUWPFVWCPO-UHFFFAOYSA-N
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General description
Application
AuNPs can be potentially used in:
- biomedical applications as a drug releasing agents[2]
- cancer radiotherapy due to their excellent surface chemistry and biocompatibility[6][7]
- electrochemical bio-sensing systems[3]
Ideal for adsorption of thiolated ligands such as oligonucleotides and polyethylene glycols. Also, due to the increased stability, these gold nanoparticles are ideal for use in physical and optical applications. Note: This product is not ideal for protein adsorption. For gold nanoparticles suitable for adsorption of proteins and other ligands for conjugate development, please see our gold nanoparticles stabilized in PBS.
This material is highly monodisperse (<12% variability in size and shape), and provides significantly improved surface reactivity. Applications include Surface Enhanced Raman Lables, Sensing/Detection, Biological Targeting, Plasmonics and Electronics.
This material is highly monodisperse (<12% variability in size and shape), and provides significantly improved surface reactivity. Applications include Surface Enhanced Raman Lables, Sensing/Detection, Biological Targeting, Plasmonics and Electronics.
Legal Information
Product of CytoDiagnostics, Inc.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
Steven J. Oldenburg, Ph.D. provides an overview of lateral flow diagnostic assays and discusses the use of ultra-bright reporter particles based on the unique optical properties of gold nanoshells that significantly increase the sensitivity of lateral flow immunoassays.
Steven J. Oldenburg, Ph.D. provides an overview of lateral flow diagnostic assays and discusses the use of ultra-bright reporter particles based on the unique optical properties of gold nanoshells that significantly increase the sensitivity of lateral flow immunoassays.
Sustainable energy sources with high production efficiency are crucial for meeting increasing energy demand.
Steven J. Oldenburg, Ph.D. provides an overview of lateral flow diagnostic assays and discusses the use of ultra-bright reporter particles based on the unique optical properties of gold nanoshells that significantly increase the sensitivity of lateral flow immunoassays.
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How to calculate molarity of this Aunps solution?
1 answer-
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Hello, I want to know if I can add CTAB (cétyltriméthylammonium bromide) to this solution and expect efficient adsorption with the AuNPs without interference with the already present stabilizer ? Thanks in advance
1 answer-
There have not been previous attempts on adsorption of non-thiolated ligands to the surfactant stabilized gold nanoparticles. It is difficult to say to what extent the surfactant may influence the adsorption of CTAB to the particles.
Please test this by transferring 1 mL of gold nanoparticle suspension into a 1.5 mL centrifuge tube, centrifuge it at 17000 x g for 25-30 minutes, remove the supernatant, and resuspend the particles in a solution of water and CTAB (<1% v/v). If the sample appears to aggregate, then this will be a sign that the reagent is not compatible with the particles.
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at which concentration is the citrate buffer?
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This is a product of CytoDiagnostics, Inc. It is supplied in 0.1mg/ml citrate buffer supplemented with a proprietary stabilizing solution.
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Is autoclave distilled water okay to use to dilute gold nanoparticle?
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Autoclaved, distilled water is suitable to use as a diluent for gold nanoparticles.
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What diluent should be used to dilute gold nanoparticle?
1 answer-
This product is prepared as a susupension in a citrate buffer. It can be further diluted with water. Avoid diluting further with buffers containing other electrolyte salts such as sodium chloride. Due to the gold nanoparticle surface charge, aggregation and color changes will occur.
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How do you attach gold nanoparticles to peptides and peptide conjugates?
1 answer-
To view information on how to attach gold nanoparticles to peptides and peptide conjugates, please view the information in the reference - Rosenthal, S. J. and Wright, D. W. (Eds.). (2005), p. 91-92. NanoBiotechnology Protocols. New Jersey. Humana Press.
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Do I need to wash my gold nanoparticles?
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For most applications, our gold nanoparticles can be used without any additional washing steps. If you have a sensitive application that requires additional washing the best way to do so is by either centrifugation or filtration.
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How do you attach gold nanoparticles to oligos?
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To attach gold nanoparticles to oligos please view the information in the attached reference - Bioconjug Chem. 2011 Apr 20;22(4):794-807. Epub 2011 Mar 28.
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Which gold nanoparticle size should I choose for my application?
1 answer-
The size of gold nanoparticle to use is very depandent upon the intended application. Generally, smaller particles offer better sensitivity in applications such as immunogold labeling due to less steric hindrance and the ability to bind more gold nanoparticles to the desired target. Small gold nanoparticles are less visible than larger particles, however, which must also be taken into account.As an application example, particles with a size between 30-50nm are particularly useful for the development of rapid tests such as lateral flow assays.
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Why does my gold nanoparticle solution turn violet when I add salt containing buffer?
1 answer-
Due to repulsive forces arising from the surface charge of gold nanoparticles, an energy barrier must be overcome for individual particles to interact. When no (or small) amounts of electrolytes such as NaCl is present, this energy barrier is too strong for interaction to occur between particles. However, upon addition of NaCl this energy barrier is reduced allowing the gold nanoparticles to interact and aggregate. This aggregation causes a phenomenon called surface-plasmon coupling which changes the adsorption maximum of light to a higher wavelength resulting in a change in color of the solution.
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