MET-Biodiesel Capillary GC Column

MET-Biodiesel Capillary GC Column

The MET-Biodiesel was designed specifically for the determination of free and total glycerin in B100 biodiesel samples.

Features and benefits of this column include:

• Good peak shape and resolution for all glyceride impurities of interest
• Ability to separate glycerin in addition to mono-, di-, and triglycerides (as methyl esters) from the FAMEs
• A maximum temperature of 380 °C (isothermal) and 430 °C (programmed), exceeding the temperature limitations specified in biodiesel methods ASTM^® D6584 and EN 14105:2003
• Metal was selected as the column material because it holds up better than fused silica under the method conditions, virtually eliminating column breakage
• Integrated guard protects the analytical column from excess reagent and non-volatile compounds, extending column life with a leak-free connection

Chromatograms

The following chromatograms were generated using the MET-Biodiesel: (click to view)

Blank Containing Internal Standards Only on the MET-Biodiesel ASTM® D6584 Calibration Standard on the MET-Biodiesel, Run 1 on a New Column ASTM® D6584 Calibration Standard on the MET-Biodiesel, Run 100 on the Same Column EN 14105 Monoglyceride Standard on the MET-Biodiesel B100 Biodiesel Sample on the MET-Biodiesel

Ordering Information for GC Columns

Description	Cat. No.
MET-Biodiesel, 14 m x 0.53 mm I.D., 0.16 µm with integrated 2 m x 0.53 mm I.D. guard	28668-U

Chromatograms

Blank Containing Internal Standards Only on the MET-Biodiesel

Peak IDs
1.	Butanetriol (I.S.)
2.	Tricaprin (I.S.)

Conditions
Column:	MET-Biodiesel, 14 m x 0.53 mm I.D., 0.16 µM with integrated 2 m x 0.53 mm I.D. guard (28668-U)
Oven:	50 °C (1 min.), 15 °C/min. to 180 °C, 7 °C/min. to 230 °C, 30 °C/min. to 380 °C (10 min.)
Det.:	FID, 380 °C
Carrier Gas:	helium, 3.0 mL/min.
Injection:	1 µL, cold on-column
Sample:	Method blank consisting of Butanetriol Internal Standard (44896-U) and Tricaprin Internal Standard (44897-U), derivatized with MSTFA (394866) then diluted in heptane

ASTM® D6584 Calibration Standard on the MET-Biodiesel, Run 1 on a New Column

Peak IDs
1.	Glycerin
2.	Butanetriol (I.S.)
3.	Monoolein
4.	Tricaprin (I.S.)
5.	Diolein
6.	Triolein

Conditions
Column:	MET-Biodiesel, 14 m x 0.53 mm I.D., 0.16 µM with integrated 2 m x 0.53 mm I.D. guard (28668-U)
Oven:	50 °C (1 min.), 15 °C/min. to 180 °C, 7 °C/min. to 230 °C, 30 °C/min. to 380 °C (10 min.)
Det.:	FID, 380 °C
Carrier Gas:	helium, 3.0 mL/min.
Injection:	1 µL, cold on-column
Sample:	ASTM® D6584 Standard Solution 1 (44899-U) plus Butanetriol Internal Standard (44896-U) and Tricaprin Internal Standard (44897-U), derivatized with MSTFA (394866) then diluted in heptane

ASTM® D6584 Calibration Standard on the MET-Biodiesel, Run 100 on the Same Column

Peak IDs
1.	Glycerin
2.	Butanetriol (I.S.)
3.	Monoolein
4.	Tricaprin (I.S.)
5.	Diolein
6.	Triolein

Conditions
Column:	MET-Biodiesel, 14 m x 0.53 mm I.D., 0.16 µM with integrated 2 m x 0.53 mm I.D. guard (28668-U)
Oven:	50 °C (1 min.), 15 °C/min. to 180 °C, 7 °C/min. to 230 °C, 30 °C/min. to 380 °C (10 min.)
Det.:	FID, 380 °C
Carrier Gas:	helium, 3.0 mL/min.
Injection:	1 µL, cold on-column
Sample:	ASTM® D6584 Standard Solution 1 (44899-U) plus Butanetriol Internal Standard (44896-U) and Tricaprin Internal Standard (44897-U), derivatized with MSTFA (394866) then diluted in heptane

EN 14105 Monoglyceride Standard on the MET-Biodiesel

Peak IDs
1.	Butanetriol (I.S.)
2.	Monopalmitin (C16:0)
3.	Monoolein (C18:1)
4.	Monostearin (C18:0)
5.	Tricaprin (I.S.)

Conditions
Column:	MET-Biodiesel, 14 m x 0.53 mm I.D., 0.16 µM with integrated 2 m x 0.53 mm I.D. guard (28668-U)
Oven:	50 °C (1 min.), 15 °C/min. to 180 °C, 7 °C/min. to 230 °C, 30 °C/min. to 380 °C (10 min.)
Det.:	FID, 380 °C
Carrier Gas:	helium, 3.0 mL/min.
Injection:	1 µL, cold on-column
Sample:	EN 14105:2003 Monoglyceride Stock Solution (49446-U) plus Butanetriol Internal Standard (44896-U) and Tricaprin Internal Standard (44897-U), derivatized with MSTFA (394866) then diluted in heptane

B100 Biodiesel Sample on the MET-Biodiesel

Peak IDs
1.	Glycerin
2.	Butanetriol (I.S.)
3.	Monopalmitin (C16:0)
4.	Monoolein (C18:1), Monolinolein (C18:2), Monolinolenin (C18:3)
5.	Monostearin (C18:0)
6.	Tricaprin (I.S.)

Conditions
Column:	MET-Biodiesel, 14 m x 0.53 mm I.D., 0.16 µM with integrated 2 m x 0.53 mm I.D. guard (28668-U)
Oven:	50 °C (1 min.), 15 °C/min. to 180 °C, 7 °C/min. to 230 °C, 30 °C/min. to 380 °C (10 min.)
Det.:	FID, 380 °C
Carrier Gas:	helium, 3.0 mL/min.
Injection:	1 µL, cold on-column
Sample:	B100 Biodiesel plus Butanetriol Internal Standard (44896-U) and Tricaprin Internal Standard (44897-U), derivatized with MSTFA (394866) then diluted in heptane