HPLC Analysis of mPEG and mPEG-Peptide (Methoxy-PEG-Maleimide) on Zenix® and Zenix®-C SEC
CONDITIONS
column
Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777033)
column
Zenix-C SEC-300, 30 cm x 7.8 mm, I.D., 3 μm particles (Z777092)
mobile phase
150 mM sodium phosphate buffer, pH 7.0
flow rate
1.0 mL/min
column temp.
25 °C
detector
UV, 214 nm
injection
20 μL
sample
20kD peptide mPEG-MAL (methoxy-PEG-Maleimide), 1 mg/mL
Descrizione
Note legali
Zenix is a registered trademark of Sepax Technologies