HPLC Analysis of Lipoxidase on TSKgel^® Phenyl-5PW

column

TSKgel Phenyl-5PW, 7.5 cm × 7.5 mm I.D., 10 μm particles (807573)

mobile phase

[A] 0.1M phosphate buffer, pH 7.0 + 1.5M ammonium sulfate; [B] 0.1M phosphate buffer, pH 7.0;

gradient

linear, 0% B to 100% B over 60 min

flow rate

0.5 mL/min

detector

UV, 280 nm