Silver, zinc oxide and titanium dioxide nanoparticle ecotoxicity to bioluminescent Pseudomonas putida in laboratory medium and artificial wastewater.

Silver, zinc oxide and titanium dioxide nanoparticle ecotoxicity to bioluminescent Pseudomonas putida in laboratory medium and artificial wastewater.

Environmental pollution (Barking, Essex : 1987) (2014-09-28)

Florian Mallevre, Teresa F Fernandes, Thomas J Aspray

ABSTRACT

Bacteria based ecotoxicology assessment of manufactured nanoparticles is largely restricted to Escherichia coli bioreporters in laboratory media. Here, toxicity effects of model OECD nanoparticles (Ag NM-300K, ZnO NM-110 and TiO2 NM-104) were assessed using the switch-off luminescent Pseudomonas putida BS566::luxCDABE bioreporter in Luria Bertani (LB) medium and artificial wastewater (AW). IC50 values ∼4 mg L(-1), 100 mg L(-1) and >200 mg L(-1) at 1 h were observed in LB for Ag NM-300K, ZnO NM-110 and TiO2 NM-104, respectively. Similar results were obtained in AW for Ag NM-300K (IC50∼5 mg L(-1)) and TiO2 NM-104 (IC50>200 mg L(-1)) whereas ZnO NM-110 was significantly higher (IC50>200 mg L(-1)). Lower ZnO NM-110 toxicity in AW compared to LB was associated with differences in agglomeration status and dissolution rate. This work demonstrates the importance of nanoecotoxicological studies in environmentally relevant matrices.

MATERIALI

N° Catalogo

Marchio

Descrizione del prodotto

205532

Sigma-Aldrich

Zinc oxide, ReagentPlus^®, powder, <5 μm particle size, 99.9%

718467

Sigma-Aldrich

Titanium(IV) oxide, nanopowder, 21 nm primary particle size (TEM), ≥99.5% trace metals basis

637254

Sigma-Aldrich

Titanium(IV) oxide, anatase, nanopowder, <25 nm particle size, 99.7% trace metals basis

544906

Sigma-Aldrich

Zinc oxide, nanopowder, <100 nm particle size

96479

Sigma-Aldrich

Zinc oxide, puriss. p.a., ACS reagent, ≥99.0% (KT)

735825

Sigma-Aldrich

Silver, conductive paste

232033

Sigma-Aldrich

Titanium(IV) oxide, anatase, powder, 99.8% trace metals basis

14021

Sigma-Aldrich

Titanium(IV) oxide, ReagentPlus^®, ≥99%

576832

Sigma-Aldrich

Silver, nanopowder, <100 nm particle size, contains PVP as dispersant, 99.5% trace metals basis

224227

Sigma-Aldrich

Titanium(IV) oxide, rutile, powder, <5 μm, ≥99.9% trace metals basis

677450

Sigma-Aldrich

Zinc oxide, nanopowder, <50 nm particle size (BET), >97%

248576

Sigma-Aldrich

Titanium(IV) oxide, anatase, powder, −325 mesh, ≥99% trace metals basis

637262

Sigma-Aldrich

Titanium(IV) oxide, rutile, nanopowder, <100 nm particle size, 99.5% trace metals basis

327085

Sigma-Aldrich

Silver, powder, 2-3.5 μm, ≥99.9% trace metals basis

721077

Sigma-Aldrich

Zinc oxide, dispersion, nanoparticles, <100 nm particle size (TEM), ≤40 nm avg. part. size (APS), 20 wt. % in H₂O

634662

Sigma-Aldrich

Titanium(IV) oxide, mixture of rutile and anatase, nanopowder, <100 nm particle size (BET), 99.5% trace metals basis

255750

Sigma-Aldrich

Zinc oxide, 99.99% trace metals basis

327077

Sigma-Aldrich

Silver, flakes, 10 μm, ≥99.9% trace metals basis

348791

Sigma-Aldrich

Titanium, foil, thickness 0.127 mm, 99.7% trace metals basis

366994

Sigma-Aldrich

Titanium, powder, <45 μm avg. part. size, 99.98% trace metals basis

14439

Sigma-Aldrich

Zinc oxide, puriss., meets analytical specification of Ph. Eur., BP, USP, 99-100.5% (calc. for dried substance)

739421

Sigma-Aldrich

Silver nanowires, diam. × L 50 nm × 6 μm, 0.5% (isopropyl alcohol suspension)

267503

Sigma-Aldrich

Titanium, foil, thickness 0.25 mm, 99.7% trace metals basis

484059

Sigma-Aldrich

Silver, nanopowder, <150 nm particle size, 99% trace metals basis

700347

Sigma-Aldrich

Titanium(IV) oxide, mixture of rutile and anatase, nanoparticles, <150 nm particle size (volume distribution, DLS), dispersion, 40 wt. % in H₂O, 99.5% trace metals basis

327026

Sigma-Aldrich

Silver, wire, diam. 0.5 mm, 99.9% trace metals basis

778095

Sigma-Aldrich

Silver nanowires, diam. × L 200 nm × 10 μm, 0.5% (isopropyl alcohol suspension)

327093

Sigma-Aldrich

Silver, powder, 5-8 μm, ≥99.9% trace metals basis

268496

Sigma-Aldrich

Titanium, powder, −100 mesh, 99.7% trace metals basis

204757

Sigma-Aldrich

Titanium(IV) oxide, rutile, ≥99.98% trace metals basis