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  • Genotyping of human papillomavirus in paraffin embedded cervical tissue samples from women in Ethiopia and the Sudan.

Genotyping of human papillomavirus in paraffin embedded cervical tissue samples from women in Ethiopia and the Sudan.

Journal of medical virology (2012-11-20)
Ebba Abate, Abraham Aseffa, Muntasir El-Tayeb, Ibrahim El-Hassan, Lawrence Yamuah, Wude Mihret, Liku Bekele, Senait Ashenafi, Nadia El-Dawi, Meseret Belayneh, Ahmed El-Hassan, Howard Engers
ABSTRACT

Cervical cancer is the most frequent female malignancy in most developing countries. Previous studies have demonstrated a strong association of human papillomavirus (HPV) infection with dysplasia and carcinoma of the uterine cervix. The objective of this study was to identify the prevailing HPV genotypes responsible for the development of cervical cancer among women in Ethiopia and the Sudan. A molecular characterization of HPV was done on 245 paraffin embedded cervical biopsy samples collected from the two countries. Amplification of HPV and subsequent genotyping was done using SPF10 primers and Line probe assay. Of samples collected from Ethiopian patients, 93% (149/160) and 13% (21/160) had high risk and low risk HPV genotypes, respectively. Among samples collected from the Sudan, 94% (80/85) harbored high risk and 11.7% (10/85) low risk HPV genotypes. Human papillomavirus 16 was the most frequent genotype identified in samples from Ethiopia (91%, 136/149) and the Sudan (82.5%, 66/80). HPV 52, 58, and 18 were the second, third and fourth common genotypes identified in Ethiopia, whereas HPV 18, 45, and 52 were the second, third, and fourth genotypes identified in samples collected from the Sudan. Thus, individuals living in different geographical localities should receive vaccines based on the specific genotypes circulating in the area and a vaccine targeting HPV 16, 18, 45, 52, and 58 may be optimal for the control of cervical cancer in the two countries.

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Paraffin wax, mp 53-58 °C (ASTM D 87)
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Paraffin wax, mp 58-62 °C (ASTM D 87)
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Paraffin wax, pellets, white
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Paraffin wax, mp ≥65 °C (ASTM D 87)
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Paraffin wax, chunks (large), white
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Paraffin wax, meets analytical specification of Ph. Eur., pastilles, white
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Paraffin wax, pellets, white, tested according to Ph. Eur.
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Paraffin wax, tested according to Ph. Eur., low viscosity
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Paraffin wax, tested according to Ph. Eur., high viscosity