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Merck

Identification of inhibitors of protein kinase B using fragment-based lead discovery.

Journal of medicinal chemistry (2007-04-25)
Gordon Saxty, Steven J Woodhead, Valerio Berdini, Thomas G Davies, Marcel L Verdonk, Paul G Wyatt, Robert G Boyle, David Barford, Robert Downham, Michelle D Garrett, Robin A Carr
ABSTRACT

Using fragment-based screening techniques, 5-methyl-4-phenyl-1H-pyrazole (IC50 80 microM) was identified as a novel, low molecular weight inhibitor of protein kinase B (PKB). Herein we describe the rapid elaboration of highly potent and ligand efficient analogues using a fragment growing approach. Iterative structure-based design was supported by protein-ligand structure determinations using a PKA-PKB "chimera" and a final protein-ligand structure of a lead compound in PKBbeta itself.

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Sigma-Aldrich
4-Pyrazoleboronic acid pinacol ester, 97%