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Key Documents

ZRB2132

Sigma-Aldrich

Anti-Ago 1 Antibody, clone 1L3 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Sinonimo/i:

Protein argonaute-1;Argonaute1;hAgo1;Argonaute RISC catalytic component 1;Eukaryotic translation initiation factor 2C 1;eIF-2C1;eIF2C1;Putative RNA-binding protein Q99

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.43

Origine biologica

rabbit

Livello qualitativo

Ricombinante

expressed in HEK 293 cells

Coniugato

unconjugated

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

1L3, recombinant monoclonal

Descrizione

1L3 Clone

Nome Commerciale

ZooMAb® learn more

Forma fisica

lyophilized

PM

calculated mol wt 97.21 kDa
observed mol wt ~105 kDa

Purificato mediante

using Protein A

Reattività contro le specie

monkey, human, mouse

Reattività contro le specie (prevista in base all’omologia)

bovine, sheep, feline

Confezionamento

antibody small pack of 25 μL

Caratteristiche più verdi

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

Convalida avanzata

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

tecniche

affinity binding assay: suitable
immunohistochemistry: suitable
western blot: suitable

Isotipo

IgG

Sequenza dell’epitopo

Internal

N° accesso ID proteina

N° accesso UniProt

Categoria alternativa più verde

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... AGO1(26523)

Descrizione generale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificità

Clone 1L3 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Protein argonaute-1 (Ago 1). It targets an epitope within 21 amino acids from the internal region.

Immunogeno

KLH-conjugated linear peptide corresponding to 21 amino acids from the internal region of human Protein argonaute-1 (Ago 1).

Applicazioni

Quality Control Testing

Evaluated by Western Blotting in NIH3T3 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ago 1 in NIH3T3 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Ago 1 in K562 and COS-7 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Ago 1 in human placenta tissue sections.

Affinity Binding Assay:: A representative lot of this antibody bound Ago1 peptide with a KD of 3.1 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in NIH3T3 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ago 1 in NIH3T3 cell lysate.

Descrizione del bersaglio

Protein argonaute-1 (UniProt: Q9UL18; also known as Argonaute1, hAgo1, Argonaute RISC catalytic component 1, Eukaryotic translation initiation factor 2C 1, eIF-2C 1, eIF2C 1, Putative RNA-binding protein Q99) I encoded by the AGO1 (also known as EIF2C1) gene (Gene ID: 26523) in human. The highly conserved Argonaute proteins play a critical role in the regulation of gene expression and orchestrate the establishment and the maintenance of cell identity throughout the entire life cycle. In humans, four functional Argonaute proteins (Ago 1-4) have been described that exhibit high structure similarity, but also have a few non-conserved amino acids in their functional domains. In contrast to Ago 2, which has two N-terminal motifs that are required for its full catalytic activity, Ago1 contains only one N-terminal motif and Ago 3 and 4 lack these motifs and are catalytically inactive. About 80% of Ago1 clusters are associated with cell-type-specific transcriptional enhancers. Ago1 is required for RNA-mediated gene silencing. It binds to short RNAs such as miRNA or siRNA and represses the translation of mRNAs which are complementary to them. Its PIWI domain is localized in amino acids 515 to 816. Ago1 lacks RNA cleavage activity due to the absence of the conserved histidine at position 805. Ago1 is shown to be enriched around transcription start sites and binds preferentially to active transcriptional enhancers. Its association with enhancers is mediated by RNAs that are transcribed from these enhancers. However, the interaction of Ago1 with enhancers does not appear to regulate transcription of the neighboring genes. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Müller, M., et al. (2020). Front. Cell Dev. Biol. 7; 360; Allo, M., et al. (2014). Proc. Natl. Acad. Sci. USA. 111(44); 15622-15629).

Stato fisico

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Ricostituzione

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stoccaggio e stabilità

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Note legali

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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