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T7024

Sigma-Aldrich

N,N,N′,N′-tetrametil-etilendiammina

BioReagent, for molecular biology, ≥99% (GC)

Sinonimo/i:

1,2-bis(dimetilamino)etano, TEMED, TMEDA

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About This Item

Formula condensata:
(CH3)2NCH2CH2N(CH3)2
Numero CAS:
110-18-9
Peso molecolare:
116.20
Beilstein:
1732991
Numero CE:
203-744-6
Numero MDL:
MFCD00008335
Codice UNSPSC:
41105319
ID PubChem:
24900242
NACRES:
NA.31

Grado

for molecular biology

Nome Commerciale

BioReagent

Saggio

≥99% (GC)

Limite di esplosione

9.08 %

Indice di rifrazione

n20/D 1.4179 (lit.)

P. eboll.

120-122 °C (lit.)

Punto di fusione

−55 °C (lit.)

Densità

0.775 g/mL at 20 °C (lit.)

Attività estranea

DNase, RNase, protease, none detected

Stringa SMILE

CN(C)CCN(C)C

InChI

1S/C6H16N2/c1-7(2)5-6-8(3)4/h5-6H2,1-4H3
KWYHDKDOAIKMQN-UHFFFAOYSA-N

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Applicazioni

This product is designated as molecular biology grade and is suitable for polyacrylamide gel electrophoresis (PAGE). It has been analyzed for the absence of nucleases and proteases.
TEMED is a tertiary amine that is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. TEMED-generated free radicals cause the compounds acrylamide and bis-acrylamide to polymerize and form the gel matrix polyacrylamide. Because TEMED is functional only in its free base form, low pH conditions will inhibit polymerization.,,
The potential contribution of TEMED to elevated background in silver stained SDS PAGE has been studied. TEMED has been used in the coating of soft polymers, such as polyacrylamide, in capillaries for investigation by atomic force microscopy. Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED.
The capillary isoelectric focusing of proteins with an isoelectric point (pI) > 12 has been investigated through the use of TEMED. TEMED has been used in the HPLC of small organic molecules.

Quantità

Suggested use is 35 μl for 50 ml acrylamide gel solution.

Avvertenze

Danger

Indicazioni di pericolo

H225,H302,H314,H331

Classi di pericolo

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B

Codice della classe di stoccaggio

3 - Flammable liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

61.7 °F - closed cup

Punto d’infiammabilità (°C)

16.5 °C - closed cup

Dispositivi di protezione individuale

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Sigma-Aldrich

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Sigma-Aldrich

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Sigma-Aldrich

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Andrews, A. T.
Electrophoresis: Theory, Techniques, and Biochemical and Clinical Applications, 21-24 (1986)
A V Vasiliskov et al.
BioTechniques, 27(3), 592-594 (1999-09-18)
The manufacturing of microchips containing oligonucleotides and proteins immobilized within gel pads, ranging in size from 10 x 10 to 100 x 100 microns, is described. The microchips are produced by photo- or persulfate-induced copolymerization of unsaturated derivatives of biomolecules
R Barberi et al.
Journal of chromatography. B, Biomedical applications, 683(1), 3-13 (1996-08-09)
Atomic force microscopy (AFM) has been used to probe the surface of a capillary after coating with "soft" polymers, notably polyacrylamides. The aim was the investigation of the efficiency of coverage of the silica surface, so as to reduce or
Sambrook, J. and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 5-5 (2001)
Arvind Chandrasekaran et al.
Lab on a chip, 19(17), 2786-2798 (2019-07-25)
To study respiratory diseases, in vitro airway epithelial models are commonly implemented by culturing airway cells on a porous surface at an air-liquid interface (ALI). However, these surfaces are often supraphysiologically stiff, which is known to affect the organization, maturation
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