R4777
Anti-Ran antibody,Mouse monoclonal
clone ARAN1, purified from hybridoma cell culture
Sinonimo/i:
Anti-ARA24, Anti-Gsp1, Anti-TC4
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About This Item
Prodotti consigliati
Origine biologica
mouse
Coniugato
unconjugated
Forma dell’anticorpo
purified from hybridoma cell culture
Tipo di anticorpo
primary antibodies
Clone
ARAN1, monoclonal
Forma fisica
buffered aqueous solution
Reattività contro le specie
human, Xenopus, bovine, mouse, hamster
tecniche
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using total extract of Jurkat cells
Isotipo
IgG2b
N° accesso UniProt
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... RAN(5901)
mouse ... Ran(19384)
Categorie correlate
Descrizione generale
Monoclonal Anti-Ran (mouse IgG2b isotype) is derived from the ARAN1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BDF1 mice immunized with denatured recombinant human Ran. Ran is a small guanine trinucleotide phosphatase (GTPase) of the Ras superfamily that like other GTPases exist in two states: bound to guanine trinucleotide phosphate (GTP) or to guanine dinucleotide phosphate (GDP). The bound form is localized to the nucleus.
Immunogeno
denatured recombinant human Ran.
Applicazioni
Monoclonal Anti-Ran antibody produced in mouse has been used in:
- western blotting
- immunoprecipitation
- enzyme linked immunosorbent assay (ELISA)
- immunocytochemistry
Azioni biochim/fisiol
Ran plays an important function in the directionality of nuclear transport. In this form it is important for the termination of the import reaction which is mediated by a complex of the cargo protein with importin-β and its adapter protein importin-α. The binding of RanGTP to importin-β causes the release of the cargo protein from the importin-α/importin-β complex. The import receptor (importin-β or transportin) is then exported out of the nucleus as a RanGTP complex and is released into the cytoplasm when RanGTP is converted to RanGDP by Ran-GAP1 (GTPase-activating protein). Export receptors respond in the opposite way by binding cargo with much higher affinity in the presence of RanGTP and exporting them out of the nucleus as RanGTP complexes. The exported cargo is released when Ran GAP1 converts RanGTP to RanGDP. In order to maintain the import/export cycle, Ran has to return to the nucleus. This is achieved by a small protein called nuclear transport factor 2 (NTF2) also known as p10, that binds RanGDP in the cytoplasm, and delivers it back into the nucleus. In the nucleus RanGDP encounters RanGEF (guanine nucleotide exchange factor) and undergoes nucleotide exchange to become RanGTP.
Stato fisico
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
A mass spectrometry-based approach for the identification of Kpnβ1 binding partners in cancer cells.
Michael O Okpara et al.
Scientific reports, 12(1), 20171-20171 (2022-11-24)
Karyopherin beta 1 (Kpnβ1) is the principal nuclear importer of cargo proteins and plays a role in many cellular processes. Its expression is upregulated in cancer and essential for cancer cell viability, thus the identification of its binding partners might
RNASEK is a V-ATPase-associated factor required for endocytosis and the replication of rhinovirus, influenza A virus, and dengue virus
Perreira JM, et al.
Cell Reports, 12(5), 850-863 (2015)
Masahiro Nagai et al.
FEBS letters, 585(5), 791-796 (2011-02-12)
The small GTPase Ran plays important roles in multiple aspects of cellular function. Maximal RanGAP activity is achieved with the aid of RanBP1 and/or presumably of RanBP2. Here, we show that RanBP1-knockout mice are unexpectedly viable, and exhibit male infertility
The role of Ran in nuclear function
Azuma Y and Dasso M
Current Opinion in Cell Biology, 12(3), 302-307 (2000)
Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins
Rexach M and Blobel G
Cell, 83(5), 683-692 (1995)
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