MAK518

ADP Assay Kit

sufficient for 100 fluorometric tests

• Sinonimo/i: ADP Detection Kit
• Codice UNSPSC: 12161503
• NACRES: NA.84

Proprietà

• Livello qualitativo: 100
• Metodo di rivelazione: fluorometric
• Malattie correlate: hematological disorder
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Adenosine diphosphate (ADP) is the product of ATP dephosphorylation by ATPases. ADP can be converted back to ATP by ATP synthases. ADP levels regulate several enzymes involved in intermediary metabolism. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, since these assays require measurement of ATP in the sample before conversion of ADP to ATP, if the nascent ATP concentration is significantly higher than the ADP concentration, the ATP signal will drown out the ADP signal.

Caratteristiche e vantaggi

• As low as 0.1 µM ADP can be quantified.
• Compatible with high-throughput handling systems.

Compatibilità

The kit is suitable for ADP determination in cells and other biological samples.

Principio

The ADP Assay Kit provides a convenient fluorometric means to measure ADP level even in the presence of ATP. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate is then quantified by a fluorometric method at λEx = 530 nm/λEm = 590 nm.

Informazioni sulla sicurezza

• Pittogrammi: GHS05,GHS07,GHS09
• Avvertenze: Danger
• Indicazioni di pericolo: H290,H314,H335,H411
• Consigli di prudenza: P234 - P261 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338
• Classi di pericolo: Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3
• Organi bersaglio: Respiratory system
• Codice della classe di stoccaggio: 8A - Combustible corrosive hazardous materials
• Classe di pericolosità dell'acqua (WGK): WGK 2