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Key Documents

MAK013

Sigma-Aldrich

Glucose and Sucrose Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Sinonimo/i:

Glucose and Sucrose Colorimetric/Fluorometric Assay Kit

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

impiego

sufficient for 100 colorimetric or fluorometric tests

applicazioni

cosmetics
food and beverages

Metodo di rivelazione

colorimetric
fluorometric

Malattie correlate

endocrinological disorders, diabetes

Temperatura di conservazione

−20°C

Descrizione generale

Sucrose is a disaccharide that is hydrolyzed to glucose and fructose by invertase. Glucose is the primary carbohydrate utilized for energy during cellular respiration. Upon cellular uptake, glucose can be shunted towards breakdown via glycolysis or polymerized for energy storage.

Applicazioni

Glucose and sucrose assay kit has been used to:
  • determine sucrose, aerobic glycolysis and pyruvate kinase activity
  • determine protein and sugar in strawberries
  • determine plasma insulin and glucose levels in mice
  • analyze plasma glucose and plasma lactate and to quantify glucose/sucrose.

Compatibilità

Suitable for use with various biological samples including serum, plasma, other body fluids, food, and growth media.

Principio

In this assay kit, glucose is oxidized via glucose oxidase resulting in a colorimetric (570 nm)/ fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the glucose present. To measure sucrose, invertase is added to the reaction to convert the sucrose to glucose and fructose. The free glucose can be subtracted from the total glucose to give the concentration of sucrose present. This kit has a linear range of detection between 0.2-1.0 nmole of glucose for the fluorometric assay and 2-10 nmoles of glucose for the colorimetric assay.

Prodotti correlati

Pittogrammi

Health hazard

Avvertenze

Danger

Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Aquatic Chronic 3 - Resp. Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

188.6 °F - closed cup

Punto d’infiammabilità (°C)

87 °C - closed cup


Certificati d'analisi (COA)

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