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Key Documents

L2650

Sigma-Aldrich

Lectin from Glycine max (soybean)

peroxidase conjugate, lyophilized powder

Sinonimo/i:

SBA, Soybean agglutinin

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About This Item

Codice UNSPSC:
12352202
NACRES:
NA.32

Origine biologica

Glycine max (soybean)

Coniugato

peroxidase conjugate

Saggio

85.00-100.00% (Warburg-Christian)

Forma fisica

lyophilized powder

Potenza

<80 μg per mL agglutination activity

peroxidase activity

25-100 units/mg protein

Composizione

Protein, ~95% modified Warburg-Christian

tecniche

western blot: suitable

N° accesso UniProt

P05046

Temperatura di conservazione

−20°C

Informazioni sul gene

soybean ... LE1(100818710)

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Applicazioni

General Western Blot Protocol:
  • Glycoprotein sample size: 500ng
  • Lectin Concentration: 0.1ug/ml

  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS page gel
  3. Transfer gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

Azioni biochim/fisiol

SBA is not blood group specific but has an affinity for N-acetyl-D-galactosamine.

Definizione di unità

One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0, at 20 °C.

Stato fisico

Contains sodium citrate

Nota sulla preparazione

Conjugates are prepared from affinity purified lectin.
Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Risultati analitici

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hr incubation at 25 °C.

Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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