GH1

89072610, rat brain, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• product name: GH1, 89072610
• Origine biologica: rat brain
• Modalità di accrescimento: Semi-adherent
• Cariotipo: Hypodiploid, modal no. 40.
• Morfologia: Epithelial
• Prodotti: Rat growth hormone, lactogenic hormones.
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Rat Wistar-Furth pituitary tumour

Descrizione della linea cellulare

Derived from pituitary gland of Wistar-Furth rats. The cells secrete growth hormone at a rate of 1μg/ml cell protein/day. Re-inoculation of cloned cells back to Wistar-Furth rats results in a marked increase in body weight and stimulation of linear growth in both intact and hypophysectomized animals independent of rat tumour size.

Applicazioni

Hormone studies

Terreno di coltura

Ham′s F10 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. These cells only semi-attach and may return to suspension during transit. If this has occurred on receipt of the cells pellet cells b

Altre note

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