A6007

Apotryptophanase from Escherichia coli

soluble powder, 75-150 units/mg solid

• Sinonimo/i: Tryptophanase from Escherichia coli, L-Tryptophan indole-lyase (deaminating)
• Numero CAS: 9024-00-4
• UNSPSC Code: 12352204
• NACRES: NA.54
• Numero CE: 4.1.99.1 (BRENDA, IUBMB)
• MDL number: MFCD00130539
• Specific activity: 75-150 units/mg solid
• Biological source: Escherichia coli

Proprietà

• biological source: Escherichia coli
• Quality Segment: 200
• form: soluble powder
• specific activity: 75-150 units/mg solid
• storage temp.: −20°C

Descrizione

Application

Apotryptophanase is used for the quantitative determination of pyridoxal-phosphate. Apotryptophanase, from Sigma, has been used to study pregnancy-associated PLP deficiency and vitamin B-6 deficiency .

Biochem/physiol Actions

Apotryptophanase hydrolizes tryptophan and is capable of catalyzing α,β-elimination reactions with a number of substituted amino acids, including S-methyl-, S-ethyl- and S-benzyl- L-cysteine. DTNB inactivates tryptophanase .

Other Notes

One unit releases one μg of indole from L-tryptophan in 10 min at pH 8.3 at 37 °C in the presence of 0.04 mM pyridoxal-5′--phosphate.

Informazioni sulla sicurezza

• Classe di stoccaggio: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)