MABS1697

Anti-WISP-3 Antibody, clone 13C4.1

clone 13C4.1, from mouse

• Sinonimo/i: WNT1-inducible-signaling pathway protein 3, CCN family member 6, WISP-3
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 13C4.1, monoclonal
• Reattività contro le specie: mouse, human, rat
• tecniche: immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_003871
• N° accesso UniProt: O95389
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... CCN6(8838)

Descrizione

Descrizione generale

WNT1-inducible-signaling pathway protein 3 (UniProt O95389; also known as CCN family member 6, WISP-3) is encoded by the WISP3 (also known as CCN6, LIBC, PPAC, PPRD, UNQ462/PRO790/PRO956) gene (Gene ID 8838) in human. WISP-3 is a member of the CCN (CTGF, Cyr61, NOV) family connective tissue growth modulators involved in cell growth/differentiation during various phases of development through a complex interplay with BMP/Notch/Wnt signaling pathways. WISP-3 is expressed in cells and tissues of mesenchymal origin and WISP3 gene mutations are linked to an autosomal recessive skeletal disorder known as progressive pseudorheumatoid dysplasia (PPRD), indicating its importance in skeletal growth and the maintenance of cartilage integrity. WISP-3 interacts with BMP and components of the Wnt1 signaling pathway including LRP6 and Fz8, promoting the expression of cartilage-specific proteins collagen-II and aggrecan. Both IGF1 and ROS stimulate WISP-3 expression, WISP-3 in turn binds to and blocks IGF1 secretion, preventing IGF1-induced collagen X induction, ROS accumulation, and alkaline phosphatase activity, all of which are associated with chondrocyte hypertrophy induction. Human WISP-3 is synthesized with a signal peptide sequence (a.a. 1-23), the removal of which yields the mature protein containing several protein-interacting domains, including an IGF-interacting IGFBP domain (a.a. 44-117) with a von Willbrand factor type C repeat, a Thrombospondin (TSP) type-1 domain (a.a. 208-253), and a C-terminal cystine knot-like (CTCK) domain (a.a. 268-342).

Specificità

Clone 13C4.1 targets an epitope within the WISP-3 IGFBP domain.

Immunogeno

GST-tagged recombinant human WISP-3 IGFBP domain fragment.

Applicazioni

Detect WISP-3 using this mouse monoclonal Anti-WISP-3, clone 13C4.1, Cat. No. MABS1697, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50-1,000 dilution of this antibody detected WISP-3 in human liver, kidney, and lung tissue sections.

Qualità

Evaluated by Western Blotting in rat kidney tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected WISP-3 in 10 µg of rat kidney tissue lysate.

Descrizione del bersaglio

~39 kDa observed. 36.84/36.33 kDa (human/rat mature form), 39.29/41.40 kDa (human isoform 1/2 pro-form), and 38.92 kDa (rat pro-form) calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Format: Purified

Altre note

Concentration: Please refer to lot specific datasheet.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1