MABE1067

Anti-ESET (SETDB1) Antibody, clone F0808

clone F0808, from mouse

• Sinonimo/i: Histone-lysine N-methyltransferase SETDB1, EC: 2.1.1.43, ERG-associated protein with SET domain, SET domain bifurcated 1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: F0808, monoclonal
• Reattività contro le specie: mouse
• Confezionamento: antibody small pack of 25 μg
• tecniche: ChIP: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG1κ
• N° accesso UniProt: O88974
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: mouse ... Setdb1(84505)

Descrizione

Descrizione generale

Histone-lysine N-methyltransferase SETDB1 (UniProt: O88974; also known as EC: 2.1.1.43, ERG-associated protein with SET domain, ESET, SET domain bifurcated 1, SETDB1) is encoded by the Setdb1 (also known as Eset, Kiaa0067) gene in murine species. SETDB1 is a ubiquitously expressed histone methyltransferase with multiple zinc-binding sites that specifically trimethylates lysine 9 of histone H3 immediately downstream of transcription start sites marked with H3K4me3 to establish the bivalent domain. It is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by Krüppel-associated box (KRAB) zinc-finger proteins. The H3K4/H3K9me3 signature keeps RNA polymerase II (Pol II) paused at the proximal promoter and maintains gene expression at low levels, but in a poised state that is ready for activation in lineage-committed cells. It functions mainly in euchromatin regions, thereby playing a key role in the silencing of euchromatic genes. It probably acts by forming a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone lysine 9 trimethylation. SETDB1 is reported to inhibit adipogenesis and its depletion induces preadipocyte differentiation and adipogenesis in mesenchymal stem cells and preadipocytes. Expression of Cebpa and Pparg genes that are its key targets of SETDB1 is enhanced when SETDB1 is knocked down in undifferentiated cells. Murine SETDB1 contains two Tudor domains (aa 257-320 and 347-403) and pre-SET (aa 744-817), SET (aa 820-1282), and post-SET (aa 1291-1307) domains that are required for its methyltransferase activity. (Ref.: Matsumura, Y., et al. (2015). Mol. Cell 60(4); 584-596).

Specificità

Clone F0808 specifically detects Histone-lysine N-methyltransferase SETDB1. It targets an epitope with in the internal region.

Immunogeno

His-tagged recombinant fragment corresponding to 213 amino acids from the internal region of murine SETDB1.

Applicazioni

Anti-ESET (SETDB1), clone F0808, Cat. No. MABE1067, is a highly specific mouse monoclonal antibody that targets Histone-lysine N-methyltransferase SETDB1 and has been tested for use in Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting.

Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected ESET (SETDB1) in Chromatin Immunoprecipitation applications (Matsumura, Y., et. al. (2015). Mol Cell. 60(4):584-96).

Immunoprecipitation Analysis: A representative lot immunoprecipitated ESET (SETDB1) in Immunoprecipitation applications (Matsumura, Y., et. al. (2015). Mol Cell. 60(4):584-96).

Qualità

Evaluated by Western Blotting in 129/S6/SvEv mouse embryonic stem cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected ESET (SETDB1) in 129/S6/SvEv mouse embryonic stem cell lysate.

Descrizione del bersaglio

~180/200 kDa observed; 144.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Format: Purified

Altre note

Concentration: Please refer to lot specific datasheet.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable