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Key Documents

AB5495

Sigma-Aldrich

Anti-Potassium Channel Kir6.2 Antibody

Chemicon®, from rabbit

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

affinity purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Purificato mediante

affinity chromatography

Reattività contro le specie

rat

Produttore/marchio commerciale

Chemicon®

tecniche

western blot: suitable

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... KCNJ11(3767)

Specificità

Recognizes Kir6.2 (Inward rectifier potassium channel Kir6.2). The antibody does not react with Kir6.1. The epitope specific for Kir6.2 is not present in any other known proteins.

Immunogeno

Highly purified peptide corresponding to residues 372-385 of rat Kir6.2 (Accession P70673). The immunogen sequence is identical in mouse and conserved in human (12/14).

Applicazioni

Anti-Potassium Channel Kir6.2 Antibody detects level of Potassium Channel Kir6.2 & has been published & validated for use in WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Western blotting: 1:200 using ECL on rat pancreas lysate.

Immunohistochemistry on rat pancrease sections. Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

Stato fisico

Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.025% sodium azide. Reconstitute with 50 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Stoccaggio e stabilità

Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Risultati analitici

Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Aquatic Chronic 3

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Maria S Lemak et al.
Frontiers in cellular neuroscience, 8, 255-255 (2014-09-16)
Medial temporal lobe structures are essential for memory formation which is associated with coherent network oscillations. During ontogenesis, these highly organized patterns develop from distinct, less synchronized forms of network activity. This maturation process goes along with marked changes in
Andrew G Edwards et al.
The Journal of physiology, 587(Pt 23), 5723-5737 (2009-10-07)
The female myocardium, relative to that of the male, exhibits sustained resistance to ischaemic tissue injury, a phenomenon termed sex-specific cardioprotection (SSC). SSC is dependent upon the sarcolemmal K(ATP) channel (sarcK(ATP)), and protein kinase C (PKC). Here we investigate whether
Atsuko Miura et al.
The Journal of biological chemistry, 281(8), 5246-5257 (2005-12-27)
Mutations in the hepatocyte nuclear factor (HNF)-4alpha gene cause a form of maturity-onset diabetes of the young (MODY1) that is characterized by impairment of glucose-stimulated insulin secretion by pancreatic beta-cells. HNF-4alpha, a transcription factor belonging to the nuclear receptor superfamily
Chisayo Kozuka et al.
British journal of pharmacology, 172(18), 4519-4534 (2015-07-04)
γ-Oryzanol, derived from unrefined rice, attenuated the preference for dietary fat in mice, by decreasing hypothalamic endoplasmic reticulum stress. However, no peripheral mechanisms, whereby γ-oryzanol could ameliorate glucose dyshomeostasis were explored. Dopamine D2 receptor signalling locally attenuates insulin secretion in

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