391334

BES

ULTROL^® Grade, ≥99% dry basis (titration), zwitterionic N-substituted aminosulfonic acid buffer

• Sinonimo/i: BES, Free Acid, ULTROL^® Grade, N,N- bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid
• Formula empirica (notazione di Hill): C₆H₁₅NO₅S
• Numero CAS: 10191-18-1
• Peso molecolare: 213.25
• Numero MDL: MFCD00007533
• Codice UNSPSC: 12161700
• NACRES: NA.25

Proprietà

• Livello qualitativo: 100
• Saggio: ≥99% dry basis (titration)
• Forma fisica: solid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Colore: white
• pKa (20 °C): 7.12
• Solubilità: water: 1 mg/mL
• Cationi in tracce: heavy metals: ≤1 ppm
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 15-25°C
• InChI: 1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12); AJTVSSFTXWNIRG-UHFFFAOYSA-N

Descrizione

Descrizione generale

Absorbance (100 mM, 260 nm):≤0.05.

BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: <0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pK_a of 7.12 at 20°C.

BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.

Attenzione

Toxicity: Standard Handling (A)

Ricostituzione

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.

Altre note

De Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
Chen, C.A., and Okayama, H. 1988. Biotechniques6, 632.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable