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04-932

Sigma-Aldrich

Anti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T, rabbit monoclonal

culture supernatant, clone C16T, from rabbit

Sinonimo/i:

calmodulin 1, calmodulin 1 (phosphorylase kinase, delta), phosphorylase kinase, delta subunit

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

culture supernatant

Tipo di anticorpo

primary antibodies

Clone

C16T, monoclonal

Reattività contro le specie

vertebrates

tecniche

western blot: suitable

Isotipo

IgG

N° accesso UniProt

Condizioni di spedizione

dry ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... CALM1(801)

Descrizione generale

Calmodulin Binding Protein (CBP) epitope tags are found in several E. coli expression vectors. Calmodulin (CaM), a small 17 kDa ubiquitous Ca2+ binding protein, acts as an intracellular Ca2+ receptor and transduces Ca2+ transients, usually in response to growth factors. It mediates the activity of a number of Ca2+ regulating enzymes, including protein kinases, phosphatases, nitric oxide synthases, and phosphodiesterase. Calmodulin is the principal intracellular calcium receptor. It is an acidic protein of 148 residues, and upon binding Ca2+, it in turn binds to short peptide sequences of many target proteins. Such interaction is thought to induce a conformational change on the target, resulting in its activation. Targets include the constitutively expressed nitric oxide synthases, the CaM kinases, and PP2B/Calcineurin.

Specificità

Recombinant proteins containing the calmodulin binding protein epitope tag.

Immunogeno

Epitope: CBP epitope
KLH-conjugated, synthetic peptide corresponding to the Calmodulin Binding Protein (CBP) epitope tag found in several E. coli expression vectors.

Applicazioni

Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
This Anti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T is validated for use in WB for the detection of Calmodulin Binding Protein Epitope Tag.

Qualità

Evaluated by Western Blot on lysates from transformed E. coli.
Western Blot Analysis: 1:2,000 dilution of this antibody detected a recombinant protein containing the calmodulin binding protein epitope tag in lysates from transformed E. coli.

Descrizione del bersaglio

The molecular weight is related to the tagged protein.

Linkage

Replaces: 05-932

Stato fisico

Cultured supernatant with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Risultati analitici

Control
Samples containing the calmodulin binding protein epitope tag.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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