Custom RNA Oligos

Standard quantities of single-stranded RNA Oligos for various applications.

Custom RNA Oligos Specifications

Scales	0.05, 0.2, 1, 10 & 15 µmol For larger quantities & in-vivo-grade material, see our Custom iScale Oligos product
Purification	Desalt, Cartridge, HPLC or PAGE Recommend purification for sequences >30 bases
Sequence Length	7 to 45 bases (simplexes) Inquire for feasibility over 45 bases
Modifications	Amino-Modifier C6, Amino-Modifier C7, Biotin, Cyanine 3, Cyanine 5, Cyanine 5.5, 6-FAM™, HEX™, TET™, Flipped Base, 2’-Fluoro, Inverted Abasic, 2’-OMe RNA, Phosphate, Thiol-Modifier-C6 S-S & Thiol-Modifier-C3 S-S Locked Nucleic Acid available Inquire for feasibility of Locked Nucleic Acid & other modifications
Quality Control	100% mass spectrometry
Format	Supplied dry in tubes Pooling & aliquoting available (inquire for feasibility)

Minimum Yield Guarantees

*Guarantees are for 20mers or longer. Oligos shorter than 20 bases will have fewer ODs. Microgram amounts are calculated based on a 30 μg/OD value. If the μg/OD value is less than 30, the reported μg value will be proportionally less. Phosphorothioated yields are 70% of the above stated values.

Non-Modified RNA Oligos (O.D./µg)*
Scale (µmole)	Desalt	Cartridge	HPLC	PAGE
0.05	5/150	1/30	1/30	0.5/15
0.2	12/360	3/90	2.5/75	1/30
1.0	40/1,200	12/360	13/390	5/150

*Guarantees are for 20mers or longer. Oligos shorter than 20 bases will have fewer ODs. Microgram amounts are calculated based on a 30 μg/OD value. If the μg/OD value is less than 30, the reported μg value will be proportionally less. Phosphorothioated yields are 70% of the above stated values. Post-synthesis modifications may yield 50% less than the above stated values.

Modified RNA Oligos (O.D./µg)*
Scale (µmole)	Desalt	Cartridge	HPLC	PAGE
0.05	2/60	0.4/12	0.4/12	0.2/6
0.2	5/150	1/30	1/30	0.4/12
1.0	16/480	5/150	5/150	2/60

Shipping Schedule

Scale (µmole)	Desalt	Cartridge	HPLC	PAGE
0.05, 0.2, and 1.0	5 days	5 days	6 days	8 days

For anything marked as ‘Inquire’ or if you have needs that are different from the other general specifications presented, please send a request to sirnarequest@milliporesigma.com.