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  • An immunohistochemical method for identifying fibroblasts in formalin-fixed, paraffin-embedded tissue.

An immunohistochemical method for identifying fibroblasts in formalin-fixed, paraffin-embedded tissue.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2007-12-12)
Tracy Goodpaster, Aster Legesse-Miller, Meera R Hameed, Seena C Aisner, Julie Randolph-Habecker, Hilary A Coller
ABSTRACT

Fibroblasts are critical for tissue homeostasis, and their inappropriate proliferation and activation can result in common and debilitating conditions including fibrosis and cancer. We currently have a poor understanding of the mechanisms that control the growth and activation of fibroblasts in vivo, in part because of a lack of suitable fibroblast markers. We have taken advantage of an antibody previously shown to stain stromal cells in frozen tissues (TE-7) and identified conditions in which it can be used to stain fibroblasts and myofibroblasts in the paraffin-embedded tissue samples routinely collected for pathological analysis. We show that this antibody recognizes growing and quiescent fibroblasts and myofibroblasts by immunohistochemistry, immunofluorescence, and ELISA assays. We also present its staining patterns in normal tissue samples and in breast tumors.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Monoclonal Anti-Fibroblast Surface Protein antibody produced in mouse, clone 1B10, ascites fluid
Sigma-Aldrich
IgG3, Lambda from murine myeloma, clone Y5606, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
IgM, Mouse, Mouse IgM Polyclonal Antibody control validated for use in ELISA.