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  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Chloroform, biotech. grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Pricing and availability is not currently available.
Sigma-Aldrich
Acetone, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, suitable for HPLC, ≥99.9%
Pricing and availability is not currently available.
Sigma-Aldrich
Acetone, suitable for HPLC, ≥99.9%
Pricing and availability is not currently available.
Sigma-Aldrich
N,N-Dimethylformamide, suitable for HPLC, ≥99.9%
Pricing and availability is not currently available.
Sigma-Aldrich
Chloroform, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Pricing and availability is not currently available.
Sigma-Aldrich
Diethyl ether, suitable for HPLC, ≥99.9%, inhibitor-free
Pricing and availability is not currently available.
Sigma-Aldrich
1-Octanol, suitable for HPLC, ≥99%
Pricing and availability is not currently available.
Sigma-Aldrich
Chloroform, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains amylenes as stabilizer
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, suitable for HPLC, gradient grade, suitable as ACS-grade LC reagent, ≥99.9%
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, HPLC Plus, ≥99.9%
Pricing and availability is not currently available.
Sigma-Aldrich
Chloroform, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains 0.5-1.0% ethanol as stabilizer
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, Absolute - Acetone free
Pricing and availability is not currently available.
Sigma-Aldrich
Acetone, ACS reagent, ≥99.5%
Pricing and availability is not currently available.
Sigma-Aldrich
Chloroform, contains ethanol as stabilizer, ACS reagent, ≥99.8%
Pricing and availability is not currently available.
SAFC
Formaldehyde solution, contains 10-15% methanol as stabilizer, 37 wt. % in H2O
Pricing and availability is not currently available.
Sigma-Aldrich
Chloroform, contains 100-200 ppm amylenes as stabilizer, ≥99.5%
Pricing and availability is not currently available.
Sigma-Aldrich
Chloroform, ACS spectrophotometric grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Pricing and availability is not currently available.
USP
Diethylene glycol, United States Pharmacopeia (USP) Reference Standard
Pricing and availability is not currently available.
Sigma-Aldrich
Tetrahydrofuran, inhibitor-free, suitable for HPLC, ≥99.9%
Pricing and availability is not currently available.
Supelco
Methanol, analytical standard
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N-Ethyldiisopropylamine, BASF quality, ≥98.0%
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Sodium chloride, BioUltra, for molecular biology, ≥99.5% (AT)
Pricing and availability is not currently available.
Sigma-Aldrich
N-Ethyldiisopropylamine solution, suitable for peptide synthesis, ~2 M in 1-methyl-2-pyrrolidinone
Pricing and availability is not currently available.
Sigma-Aldrich
Acetone, purum, ≥99.0% (GC)
Pricing and availability is not currently available.
Supelco
Tetrahydrofuran, Selectophore, ≥99.5%
Pricing and availability is not currently available.
Supelco
N,N-Dimethylformamide, analytical standard
Pricing and availability is not currently available.
Sigma-Aldrich
Formaldehyde solution, tested according to Ph. Eur.
Pricing and availability is not currently available.
Supelco
Chloroform, analytical standard
Pricing and availability is not currently available.
Sigma-Aldrich
Tetraethyl orthosilicate, ≥99.0% (GC)
Pricing and availability is not currently available.