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  • Flavonoid composition and antioxidant activities of Chinese local pummelo (Citrus grandis Osbeck.) varieties.

Flavonoid composition and antioxidant activities of Chinese local pummelo (Citrus grandis Osbeck.) varieties.

Food chemistry (2014-05-20)
Wanpeng Xi, Bo Fang, Qiyang Zhao, Bining Jiao, Zhiqin Zhou
ABSTRACT

China is one of the most important diversity centres of the genus Citrus L. and is particularly rich in pummelo germplasm. In this study, the flavonoids in the peels and pulps of 28 Chinese local pummelos and four grapefruits were determined by optimised Ultra Performance Liquid Chromatography (UPLC), and their antioxidant capacities were evaluated using 2,2-diphenyl-1-picrylhydrazyl radicals (DPPH), ferric reducing/antioxidant power (FRAP) and superoxide anion methods. We found that naringin was the predominant flavonoid in pummelo, while naringin and neohesperidin were the predominant flavonoids in grapefruit. The fruit peels of Citrus paradisi cvs. Rio Red and Cocktail had the highest contents of naringin (9871.69mg/kg FW) and neohesperidin (7011.15mg/kg FW), respectively. Overall, C. paradisi cvs. Cocktail, Rio Red and Changshanhuyou, Citrus grandis cvs. 28-19, Chandler, and Hongxinyou contained more flavonoids and exhibited higher antioxidant capacities and are potentially good sources of phytochemicals and natural antioxidants.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Naringenin, natural (US), 98%
Sigma-Aldrich
Acetic acid-12C2, 99.9 atom % 12C
Sigma-Aldrich
Nobiletin, ≥97%
Supelco
Acetic acid, analytical standard
Supelco
2,4,6-Tris(2-pyridyl)-s-triazine, for spectrophotometric det. of Fe, ≥99.0% (HPLC)
Sigma-Aldrich
Acetic acid, natural, ≥99.5%, FG
Sigma-Aldrich
Acetic acid, ≥99.5%, FCC, FG
Sigma-Aldrich
Eriocitrin, ≥98.0% (HPLC)
Millipore
2,3,5-Triphenyl-tetrazolium chloride solution, suitable for microbiology, Filter sterilized solution that is recommended for the detection of microbial growth based on reduction of TTC
Sigma-Aldrich
Acetic acid, for luminescence, BioUltra, ≥99.5% (GC)
Sigma-Aldrich
(±)-Naringenin, ≥95%
Supelco
(±)-Naringenin, analytical standard
Supelco
2,4,6-Tris(2-pyridyl)-s-triazine, for spectrophotometric det. (of Fe), ≥98%
Eriocitrin, primary reference standard
Sigma-Aldrich
Acetic acid, glacial, ReagentPlus®, ≥99%
Sigma-Aldrich
Acetic acid solution, suitable for HPLC
Sigma-Aldrich
Acetic acid, glacial, ≥99.99% trace metals basis
Sigma-Aldrich
Acetic acid, glacial, ACS reagent, ≥99.7%
Supelco
Residual Solvent - Acetonitrile, Pharmaceutical Secondary Standard; Certified Reference Material
Millipore
Bifido Selective Supplement B, suitable for microbiology
Supelco
Hesperidin, analytical standard
Sigma-Aldrich
Hesperetin, ≥95%
Supelco
Dimethyl sulfoxide, for inorganic trace analysis, ≥99.99995% (metals basis)
Hesperidin, primary reference standard
USP
Glacial acetic acid, United States Pharmacopeia (USP) Reference Standard
Supelco
Naringin, analytical standard
Sigma-Aldrich
Acetonitrile, electronic grade, 99.999% trace metals basis
Sigma-Aldrich
5α-Androstan-17β-ol-3-one, purum, ≥99.0% (TLC)
Sigma-Aldrich
Naringin, ≥95% (HPLC)
Supelco
Acetonitrile, analytical standard