- Nanoflow electrospinning serial femtosecond crystallography.
Nanoflow electrospinning serial femtosecond crystallography.
Acta crystallographica. Section D, Biological crystallography (2012-10-24)
Raymond G Sierra, Hartawan Laksmono, Jan Kern, Rosalie Tran, Johan Hattne, Roberto Alonso-Mori, Benedikt Lassalle-Kaiser, Carina Glöckner, Julia Hellmich, Donald W Schafer, Nathaniel Echols, Richard J Gildea, Ralf W Grosse-Kunstleve, Jonas Sellberg, Trevor A McQueen, Alan R Fry, Marc M Messerschmidt, Alan Miahnahri, M Marvin Seibert, Christina Y Hampton, Dmitri Starodub, N Duane Loh, Dimosthenis Sokaras, Tsu-Chien Weng, Petrus H Zwart, Pieter Glatzel, Despina Milathianaki, William E White, Paul D Adams, Garth J Williams, Sébastien Boutet, Athina Zouni, Johannes Messinger, Nicholas K Sauter, Uwe Bergmann, Junko Yano, Vittal K Yachandra, Michael J Bogan
PMID23090408
ABSTRACT
An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14-3.1 µl min(-1) to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min(-1) and diffracted to beyond 4 Å resolution, producing 14,000 indexable diffraction patterns, or four per second, from 140 µg of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Thermolysin from Geobacillus stearothermophilus, Type X, lyophilized powder, 30-350 units/mg protein (E1%/280)
Sigma-Aldrich
Thermolysin from Geobacillus stearothermophilus, powder, BioReagent, 30-350 units/mg protein (E1%/280), suitable for cell culture