- Neural induction with a dopaminergic phenotype from human pluripotent stem cells through a feeder-free floating aggregation culture.
Neural induction with a dopaminergic phenotype from human pluripotent stem cells through a feeder-free floating aggregation culture.
Methods in molecular biology (Clifton, N.J.) (2013-05-18)
Asuka Morizane, Daisuke Doi, Jun Takahashi
PMID23681613
ABSTRACT
Pluripotent stem cells are promising potential sources for cell replacement therapy and are useful research tools for exploring disease mechanisms. Neural cells are one of the cell types that have been most efficiently differentiated through several established protocols. This chapter describes the feeder-free floating aggregation culture system for the induction of dopaminergic neurons. This method is simple and highly efficient for the production of dopaminergic neurons. It has several advantages for application in clinical usage in comparison to the other protocols using either feeder cells or Matrigel.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
N6,2′-O-Dibutyryladenosine 3′,5′-cyclic monophosphate sodium salt, ≥97% (HPLC), powder
Sigma-Aldrich
Purmorphamine, A cell-permeable activator of Hedgehog signaling that induces osteoblast differentiation of multipotent mesenchymal progenitor cells C3H10T1/2 (EC₅₀ = 1 µM).