M7769
IgG2a, Kappa from murine myeloma
clone UPC 10, ascites fluid, lyophilized powder
Synonym(s):
Mouse IgG2a-κ
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About This Item
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
clone
UPC 10, monoclonal
form
lyophilized powder
storage temp.
2-8°C
target post-translational modification
unmodified
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General description
The UPC-10 tumor line that produces mouse IgG2a, K is a tetramethylpentadecane (pristane) induced plasmacytoma originated and carried intraperitoneally in BALB/c mice. The hapten binding specificity of the UPC-10 line is for β-2-6-linked fructosan. The ascites produced from the UPC-10 tumor line contains IgG2a, K in addition to normal levels of other mouse immunoglobulins and serum proteins. The ascites fluid is clarified by centrifugation and filtration. IgG2a (immunoglobulin G 2a) is one of the subclasses of rat immunoglobulins, which also include IgG1, IgG2b and IgG2c.
Specificity
Antigen Specificity: β-2,6-fructosan
Trace amounts of lambda chain may be present.
Trace amounts of lambda chain may be present.
Immunogen
The UPC-10 tumor line that produces mouse IgG2a, K is a tetramethylpentadecane (pristane) induced plasmacytoma originated and carried intraperitoneally in BALB/c mice.
Application
IgG2a, Kappa from murine myeloma has been used in immunofluorescence and immunohistochemistry.
Biochem/physiol Actions
The IgG2a (immunoglobulin G 2a) plays a vital role in stimulating autoimmunity in mice. This gene is expressed at high level during virus infection. IgG2a offers host defence through activation of complement system. This antibody has specificity towards Fc receptors expressed on phagocytes and stimulates antibody-dependent cell mediated cytotoxicity. IgG2a also induces macrophage-mediated opsonophagocytosis, which has been observed in response to influenza virus and leads to its clearance from the infected cells.
Physical form
The product provided is lyophilized from 0.01 M phosphate buffered saline, pH 7.2, with no preservatives added.
Preparation Note
Storage and Stability: Store the product at 2-8 °C. After reconstitution, the solution may be stored frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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N Reddy et al.
Cancer immunology, immunotherapy : CII, 46(1), 25-33 (1998-03-31)
We reported previously that the blood clearance of injected mouse IgG2a was extremely rapid in many strains of nude and nu/+ mice. In an attempt to determine the cause of this phenomenon, the levels of endogenous IgG2a in the blood
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HAP2 is a transmembrane gamete fusogen found in multiple eukaryotic kingdoms and is structurally homologous to viral class II fusogens. Studies in Plasmodium have suggested that HAP2 is an attractive target for vaccines that block transmission of malaria. HAP2 has
O Tabary et al.
Journal of immunology (Baltimore, Md. : 1950), 164(6), 3377-3384 (2000-03-08)
Increasing evidence suggests that in airways from cystic fibrosis (CF) patients, inflammation may precede bacterial infection and be related to an endogenous dysregulation of proinflammatory cytokines in airway epithelial cells. Several investigators have reported that, in CF airway fluids, elevated
S Brézillon et al.
The Journal of biological chemistry, 272(44), 27830-27838 (1997-11-05)
To mimic the effect of ischemia on the integrity of airway epithelium and expression of cystic fibrosis transmembrane conductance regulator (CFTR), we induced an ATP depletion of the respiratory epithelium from upper airway cells (nasal tissue) and human bronchial epithelial
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