A0483

Monoclonal Anti-Actin (20-33) antibody produced in rabbit

clone SIG2-AC2, ascites fluid

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: SIG2-AC2, monoclonal
• mol wt: antigen ~42 kDa
• species reactivity: chicken, rat, human
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; indirect ELISA: suitable; indirect immunofluorescence: 1:400; western blot: 1:50-1:100 using chicken gizzard total extract
• isotype: IgG
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ACTB(60)

Description

General description

Monoclonal Anti-Actin (20-33) (rabbit IgG) is a rabbit monoclonal derived from the hybridoma SIG2-AC2 produced by the fusion of rabbit myeloma cells and splenocytes from rabbits immunized with a synthetic peptide. Actin is one of the most conserved eukaryotic proteins and is expressed in mammals and birds with at least six isoforms characterized by electrophoresis and amino acid sequence analysis. There are three α-actins (skeletal, cardiac and smooth muscle), one β-actin (β-non-muscle) and two γ-actins (γ-smooth muscle and g-non-muscle).

Immunogen

synthetic peptide corresponding to amino acids 20-33 of actin, attached to a multiple antigen peptide (MAP) backbone.

Application

Monoclonal Anti-Actin (20-33) antibody produced in rabbit has been used in:

• western blotting
• enzyme-linked immunosorbent assay (ELISA)
• immunohistochemistry
• immunocytochemistry

Physical form

Supplied as ascites fluid containing 15 mM sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves