Skip to Content
Merck
All Photos(1)

Key Documents

14-906

Sigma-Aldrich

PI3K-C2α Protein, 10 µg

Sign Into View Organizational & Contract Pricing

Select a Size

14-906
€384.00

€384.00


Estimated to ship onApril 22, 2025Details


Request a Bulk Order

Select a Size

Change View
14-906
€384.00

About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405

€384.00


Estimated to ship onApril 22, 2025Details


Request a Bulk Order

recombinant

expressed in baculovirus infected Sf21 cells

Quality Level

description

protein target PI3K-C2α

purified by

immobilized metal affinity chromatography (IMAC)

technique(s)

activity assay: suitable

UniProt accession no.

Gene Information

human ... PIK3CG(5294)

General description

N-terminal 6His-tagged recombinant human PI3K-C2α amino acids 299–end.

Packaging

Also available in 250μg size as catalog # 14-906M - Call for pricing and availability.

Quality

Routinely evaluated in the PIProfiler.

Physical properties

161kDa

Physical form

10 μg of enzyme in 10.6 μl of 50 mM Tris/HCl, pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 0.2 mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol, 20 mM β-glycerophosphate, 0.25 mM Na3VO4, 10 mM NaF. Frozen solution.

Storage and Stability

6 months at -70°C

Analysis Note

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Other Notes

FUNCTION: Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2. May play a role in clathrin-coated endocytic vesicle formation and EGF signaling cascade. May be involved in mitosis and UV-induced damage response. May be a downstream effector in insulin signaling cascade.
CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate.
COFACTOR: Calcium or magnesium. Manganese cannot be used.
ENZYME REGULATION: Activated by insulin. Only slightly inhibited by wortmannin and LY294002. Activated by clathrin.
Subunit structure: Part of a complex with ERBB2 and EGFR. Interacts with clathrin trimers.
SUBCELLULAR LOCATION: Cell membrane. Golgi apparatus. Cytoplasmic vesicle › clathrin-coated vesicle. Nucleus. Cytoplasm. Associated with RNA-containing structures.
TISSUE SPECIFICITY: Expressed in columnar and transitional epithelia, mononuclear cells, smooth muscle cells, and endothelial cells lining capillaries and small venules (at protein level). Ubiquitously expressed, with highest levels in heart, placenta and ovary, and lowest levels in the kidney.
SIMILARITY: Belongs to the PI3/PI4-kinase family. Contains 1 C2 domain. Contains 1 PI3K/PI4K domain. Contains 1 PX (phox homology) domain.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service