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G005390

HPLC Analysis of Diet Soda Spiked with Neotame on Ascentis® Express C18

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 3 cm x 4.6 mm I.D., 2.7 μm particles (53818-U)
mobile phase: [A] 100 mM ammonium acetate, pH 5.6 (titrated with acetic acid); [B] water; [C] acetonitrile
gradient: 20% A constant; 75 to 20% B, 5 to 60% C in 1 min; held at 20% B, 60% C for 0.1 min
flow rate: 3 mL/min
pressure: 3191 psi (220 bar)
column temp.: 40 °C
detector: UV, 214 nm
injection: 1 μL
sample: neotame 350 μg/mL in diet cola

suitability

application for HPLC

application(s)

food and beverages

Analysis Note

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of common drink additives used in diet soda.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

analytical column

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