A7827
Alkaline Phosphatase-Anti-Alkaline Phosphatase antibody produced in mouse
clone AP1B9, purified immunoglobulin, buffered aqueous solution
Synonym(s):
APAAP
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
Recommended Products
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
AP1B9, monoclonal
description
Concentrate
form
buffered aqueous solution
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tonsil
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
General description
A soluble complex of calf intestinal alkaline phosphatase and mouse monoclonal antibodies to alkaline phosphatase. The product is a soluble complex of calf intestinal alkaline phosphatase and mouse monoclonal antibodies to alkaline phosphatase.
Monoclonal Anti-Alkaline Phosphatase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Application
Alkaline Phosphatase-Anti-Alkaline Phosphatase antibody produced in mouse has been used in:
- Immunohistological analysis
- Immunohistochemistry assay
- Immunocytochemical staining
Apoptosis of β-cells in mouse pancreatic sections was analyzed by incubating section with TUNEL and anti-insulin antibody. APAAP was used as the secondary antibody and BCIP/NBT (Sigma) as the substrate.
Biochem/physiol Actions
Mouse Monoclonal Alkaline Phosphatase Anti-Alkaline Phosphatase is a soluble complex formed from levamisole-resistant calf intestinal alkaline phosphatase and purified mouse monoclonal antibody to alkaline phosphatase. The use of a mouse monoclonal alkaline phosphatase anti-alkaline phosphatase (APAAP) complex, in conjunction with a primary mouse antibody and a secondary bridging antibody (anti-mouse IgG), results in an intense signal with a very low background, while avoiding problems inherent to the covalent conjugation of antibodies. The technology of unlabelled antibody peroxidase-anti peroxidase (PAP) and the alkaline phosphatase anti- alkaline phosphatase (APAAP) method has applications in immunostaining. APAAP monoclonal antibodies complex are homogenous and this method is effective and its sensitive.
Physical form
Solution in 0.05 M Tris buffered saline, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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J L Klein et al.
Bone marrow transplantation, 28(11), 1023-1029 (2002-01-10)
Fifty women with breast cancer metastatic to bone or bone marrow involvement on light microscopy at the time of initial evaluation were treated with high-dose chemotherapy (HDC) and peripheral blood progenitor cell (PBPC) transplantation with CD34(+) cell selection using the
APAAP complex: production and usage in immunocytochemical and immunohistochemical staining
Naseri M, et al.
Human Antibodies, 16(3-4), 107-115 (2007)
Tumor control in a model of bone marrow transplantation and acute liver-infiltrating B-cell lymphoma: an unpredicted novel function of cytomegalovirus
Erlach KC, et al.
Journal of Virology, 76(6), 2857-2870 (2002)
High-dose chemotherapy and CD34-selected peripheral blood progenitor cell transplantation for patients with breast cancer metastatic to bone and/or bone marrow
Klein JL, et al.
Bone Marrow Transplantation, 28(11), 1023-1023 (2001)
Montserrat Biarnés et al.
Diabetes, 51(1), 66-72 (2002-01-05)
We studied the effects of hyperglycemia on beta-cell death and mass in syngeneically transplanted islets. Six groups of STZ-induced diabetic C57BL/6 mice were transplanted with 100 syngeneic islets, an insufficient beta-cell mass to restore normoglycemia. Groups 1, 2, and 3
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