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HADK2MAG-61K

Millipore

MILLIPLEX® Human Adipokine Magnetic Bead Panel 2 - Endocrine Multiplex Assay

The Human Adipokine Magnetic Bead Panel 2, using the Luminex xMAP technology, enables the simultaneous analysis of nine protein biomarkers in human serum and plasma samples.

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47

Quality Level

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 87%
(IL-8)

accuracy: 91%
(TNFα)

accuracy: 94%
(Insulin)

standard curve range: 0.64-10,000 pg/mL
(IL-8)

standard curve range: 0.64-10,000 pg/mL
(NGF)

standard curve range: 0.64-10,000 pg/mL
(TNF-α)

standard curve range: 0.96-15,000 pg/mL
(IL-6)

standard curve range: 1.3-20,000 pg/mL
(IL-1β)

standard curve range: 1.3-20,000 pg/mL
(MCP-1)

standard curve range: 38-600,000 pg/mL
(Leptin)

standard curve range: 9.6-150,000 pg/mL
(HGF)

standard curve range: 9.6-150,000 pg/mL
(Insulin)

inter-assay cv: <15%
intra-assay cv: <10%
(IL-1β)

inter-assay cv: <15%
intra-assay cv: <10%
(IL-6)

inter-assay cv: <15%
intra-assay cv: <10%
(IL-8)

inter-assay cv: <15%
intra-assay cv: <10%
(Insulin)

inter-assay cv: <15%
intra-assay cv: <10%
(Leptin)

inter-assay cv: <15%
intra-assay cv: <10%
(MCP-1)

inter-assay cv: <15%
intra-assay cv: <10%
(NGF)

inter-assay cv: <20%
intra-assay cv: <10%
(TNFα)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

General description

Previously the role of white adipose tissue was thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism.

The MILLIPLEX® Human Adipokine Panel 2 is a 9-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum or plasma samples: HGF, IL-1β, IL-6, IL-8, Insulin, Leptin, MCP-1, NGF and TNF-α. This kit uses a 96-well format, contains a lyophilized standard cocktail, 2 quality controls and can measure up to 38 samples in duplicate.

Panel Type: Metabolism

Specificity

Cross Reactivty
Each antibody pair in a panel is highly specific and displays insignificant cross-reactivity to other analytes in the panel.

Application

  • Analytes: IL-1β, IL-6, IL-8, Insulin, Leptin, MCP-1, NGF, TNF-α
  • Recommended Sample Type: Human serum and plasma
  • Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C
  • Research Category: Metabolism
  • Research Subcategory: Endocrine

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

96-well plate
Everything you need in a single kit.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Please contact Technical Service for linearity of dilution.
Sensitivity: See kit protocol for individual sensitives.

Legal Information

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Skull and crossbonesEnvironment

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Racial differences in HbA1c: a cross-sectional analysis of a Brazilian public primary care population.
de Miranda, VA; Cruz Filho, RA; de Oliveira, TS; Moscavitch, SD; Kang, HC; Miranda Chagas et al.
Primary Care Diabetes null
Maria A Ramos-Roman et al.
Diabetes, 69(8), 1624-1635 (2020-05-10)
Milk production may involve a transient development of insulin resistance in nonmammary tissues to support redistribution of maternal macronutrients to match the requirements of the lactating mammary gland. In the current study, adipose and liver metabolic responses were measured in
Hwallip Bae et al.
Neuropsychiatric disease and treatment, 10, 2153-2157 (2014-11-25)
The role of neurotrophic factors has been highlighted as a cause of decline in the cognitive function of alcohol-dependent patients. It is known that nerve-growth factor (NGF), one of the neurotrophins, is related to the growth and differentiation of nerve
Altered promoter methylation of PDK4, IL1 B, IL6, and TNF after Roux-en Y gastric bypass.
Kirchner, H; Nylen, C; Laber, S; Barres, R; Yan, J; Krook, A; Zierath, JR; Naslund, E
Surgery for Obesity and Related Diseases : Official Journal of the American Society for Bariatric Surgery null
Annalisa Berzigotti et al.
Hepatology (Baltimore, Md.), 65(4), 1293-1305 (2016-12-21)
Obesity increases the risk of clinical decompensation in cirrhosis, possibly by increasing portal pressure. Whether weight reduction can be safely achieved through lifestyle (LS) changes (diet and exercise) in overweight/obese patients with cirrhosis, and if weight loss reduces portal pressure

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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