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I8648

Sigma-Aldrich

Interleukin-15 human

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

IL-15

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About This Item

MDL number:
MFCD00675504
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

human

Quality Level

100

recombinant

expressed in E. coli

Assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

0.2-4.0 ng/mL EC50

mol wt

antigen predicted mol wt ~12.5 kDa

packaging

pkg of 5 μg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P40933

storage temp.

−20°C

Gene Information

human ... IL15(3600)

Biochem/physiol Actions

IL-15 (interleukin 15) is a proinflammatory pleiotropic cytokine and is a connection between innate and adaptive immune responses. It participates in the proliferation, survival and activation of various lymphocytes lineages. IL-15 works by associating with a high affinity heterotrimeric receptor complex containing IL-15Rα, IL-2/15Rβ and IL-Rγ. It is present in different forms, the soluble form, the hyper IL-15 formed by association between IL-15 receptor α chain and soluble cytokine, and the membrane bound isoform.
Interleukin-15 was first isolated from the supernatant of a cultured simian kidney epithelial cell line. The cDNA encodes a 162 amino acid precursor protein with a 48 amino acid signal peptide that is cleaved to yield a 114 amino acid mature protein. Human IL-15 shares sequence identity with simian (~97%) and murine (~73%). IL-15 competes for binding sites with IL-2, as both IL-2 and IL-15 stimulate the growth of cells through the IL-2 receptor.

Physical form

Lyophilized from 0.2 μm filtered solution in phosphate buffered saline, pH 7.4 with 50 μg bovine serum albumin per 1 μg as a carrier protein.

Analysis Note

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The specific activity of Recombinant Human IL-15 is approximately 4.5 x 105 U/μg, which is calibrated against recombinant human IL-15 WHO International Standard (NIBSC code: 95/554).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hua Wang et al.
Medical oncology (Northwood, London, England), 32(1), 370-370 (2014-11-28)
Interleukin-15 (IL-15) is a proinflammatory cytokine involved in the proliferation, survival, and activation of multiple lymphocyte lineages. However, the prognostic significance of IL-15 for extranodal NK/T cell lymphoma (ENKTL) has not been well established. We retrospectively analyzed 112 patients with
Mario Bunse et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 22(11), 1983-1991 (2014-07-23)
Genetically modified T cells that express a transduced T cell receptor (TCR) α/β heterodimer in addition to their endogenous TCR are used in clinical studies to treat cancer. These cells express two TCR-α and two TCR-β chains that do not
K H Grabstein et al.
Science (New York, N.Y.), 264(5161), 965-968 (1994-05-13)
A cytokine was identified that stimulated the proliferation of T lymphocytes, and a complementary DNA clone encoding this new T cell growth factor was isolated. The cytokine, designated interleukin-15 (IL-15), is produced by a wide variety of cells and tissues
Jianan Zhu et al.
Frontiers in immunology, 13, 813218-813218 (2022-03-01)
Unexplained recurrent spontaneous abortion (URSA) is believed to be associated with impaired immunosuppression at the maternal-fetal interface, but the detailed molecular mechanism remains unclear. The ATP-adenosine metabolic pathway regulated by CD39/CD73 has recently been recognized to be important in immunosuppression.
Alicia Santos Savio et al.
BMC musculoskeletal disorders, 16, 51-51 (2015-04-17)
Pro-inflammatory cytokines are directly implicated in the pathogenesis of Rheumatoid arthritis (RA). Variable clinical response to cytokine targeted therapies as TNFalpha and IL-6, strongly highlights the heterogeneity of inflammatory process in RA. Another cytokine, IL-15 has also been related to
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