TLC Analysis of Alkaloids on HPTLC CN F254s
Materials
accessory
Product No.
Description
Pricing
Hamilton® TLC syringes
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
CONDITIONS
plate
HPTLC pre-coated plate CN F 254s (1.16464.0001)
eluent
[A] methanol; [B] l-propanol; [C] water; [D] ammonia solution 25% (30:20:50:1, A:B:C:D) containing 0.1 M ammonium bromide
migration distance
7 cm
chamber
normal chamber without chamber saturation
sample
0.3 μL
detector
in situ evaluation with TLC/HPTLC scanner (Camag) UV 254 nm