TLC Analysis of 4-Hydroxybenzoic Acid and 4-Hydroxybenzoic Acid Esters on HPTLC Silica Gel 60 RP-18 F254s: Effect of Eluent Stabilizer on Resolution
Materials
accessory
Product No.
Description
Pricing
Hamilton® TLC syringes
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
CONDITIONS
plate
HPTLC pre-coated plate RP-18 F 254s (1.13724.0001)
eluent
chloroform with stabilizer, 2-methyl-2-butene or ethanol as shown
migration distance
7 cm
chamber
normal chamber without chamber saturation
sample
0.2 μL
detector
in situ evaluation with TLC/HPTLC scanner (Camag) UV 254 nm