GC Analysis of Blood Alcohols in Human Plasma on SUPELCOWAX 10 after SPME using 60 μm Carbowax^® Fiber

sample/matrix

blood alcohols at concentrations indicated in human plasma

SPME fiber

60 μm Carbowax

extraction

headspace, 50 °C (5 min)

desorption process

220 °C, 2 min

column

SUPELCOWAX 10, 30 m × 0.25 mm I.D., 0.50 μm (24284)

oven

35 °C (2 min), 10 °C/min to 125 °C (1 min)

detector

FID, 200 °C

carrier gas

helium, 1.0 mL/min constant

injection

10:1 split

liner

0.75 mm I.D. SPME liner