GC Analysis of Aroma Fusel Alcohols in Grappa Sample 2 on Watercol™ 1910 after Headspace SPME using 75 μm Carboxen®/PDMS Fiber, MSD
Materials
analytical column
CONDITIONS
sample preparation
Solid Phase Microextraction
sample/matrix
0.5 mL grappa sample 2 in a 2 mL vial
SPME fiber
75 μm Carboxen®/polydimethylsiloxane on a fused silica core (57318)
extraction
headspace, 60 °C for 45 min
desorption process
300 °C for 1.1 min
column
Watercol 1910, 30 m x 0.25 mm I.D., 0.20 μm ; two identical columns installed in the same inlet, each going to a separate detector (29711-U)
oven
40 °C (2 min), 4 °C/min to 125 °C (1 min)
inj. temp.
300 °C
detector
MSD, m/z = 18-500
MSD interface
260 °C
carrier gas
helium, 1.1 mL/min
injection
splitless
liner
0.75 mm I.D., direct (SPME) type, straight design (2633901)
Description
Analysis Note
Fusel alcohols are by-products of the fermentation process. Four of these (n-propanol, isobutanol, active amyl alcohol, and isoamyl alcohol) are important to alcoholic beverage manufacturers due to their aroma characteristics. The "Watercol" series of ionic liquid capillary GC columns are characterized by their ability to produce a sharp peak shape for water, sharp enough so that water can be integrated and quantified. Sharp peak shapes are also produced for many other small polar analytes, such as the four aroma fusel alcohols. In this application, a brandy sample was analyzed for the presence of aroma fusel alcohols. As shown, great peak shapes were obtained, resulting in good resolution. In addition to sharp fusel alcohol peaks, the column′s selectivity results in elution of both ethanol and water free from all analytes.
Legal Information
Carboxen is a registered trademark of Merck KGaA, Darmstadt, Germany
Watercol is a trademark of Sigma-Aldrich Co. LLC