Application of the Fenton process in the elimination of helminth eggs.

Application of the Fenton process in the elimination of helminth eggs.

Journal of water and health (2014-12-05)

Sandra Isabel Escobar-Megchún, Hugo Alejandro Nájera-Aguilar, Magaly González-Hilerio, Javier Gutiérrez-Jiménez, Rubén Fernando Gutiérrez-Hernández, María Neftalí Rojas-Valencia

PMID25473981

ABSTRACT

This study relates to a method for evaluating the degradation efficiency of Ascaris lumbricoides eggs through a Fenton reaction, using hydrogen peroxide (H2O2) at 100, 250, and 500 mg/L, ferrous sulfate (FeSO4) at 458 mg/L Fe(2+) and pH values of 3, 4.5, and 6. The experiments were conducted according to a 3(2) experimental design, with 1:1, 3:1, and 5:1 (H2O2/Fe(2+)) molar ratios. The oxidation and flocculation stages were performed at 130 rpm during 2 hours and at 25-30 rpm during 20 min, respectively. As a result of the Fenton reaction, an average of 91.2% Ascaris lumbricoides egg degradation was achieved at pH 6 using a 500 mg/L dose of H2O2 and a 3:1 (H2O2/Fe(2+)) molar ratio. Thus, this process is an alternative for eliminating parasites that are resistant to conventional disinfection processes and significantly reduces the human health hazard they represent.

MATERIALS

Product Number

Brand

Product Description

88597

Millipore

Hydrogen peroxide solution, 3%, suitable for microbiology

44890

Sigma-Aldrich

Iron, puriss. p.a., carbonyl-Iron powder, low in magnesium and manganese compounds, ≥99.5% (RT)

IRMM524B

Iron, IRMM^®, certified reference material, 0.5 mm wire

356808

Sigma-Aldrich

Iron, foil, thickness 0.1 mm, ≥99.9% trace metals basis

209309

Sigma-Aldrich

Iron, powder, −325 mesh, 97%

267945

Sigma-Aldrich

Iron, chips, 99.98% trace metals basis

F8263

Sigma-Aldrich

Iron(II) sulfate heptahydrate, suitable for plant cell culture, ≥99%

F8633

Sigma-Aldrich

Iron(II) sulfate heptahydrate, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

215422

Sigma-Aldrich

Iron(II) sulfate heptahydrate, ACS reagent, ≥99.0%

GF63891726

Iron, insulated wire, 10m, conductor diameter 0.25mm, insulation thickness 0.01mm, polyester insulation

GF83304828

Iron, tube, 1000mm, outside diameter 25.0mm, inside diameter 21mm, wall thickness 2mm, annealed, 99.5%

GF27258329

Iron, tube, 1000mm, outside diameter 12.0mm, inside diameter 9mm, wall thickness 1.5mm, annealed, 99.5%

GF33572606

Iron, tube, 500mm, outside diameter 5.0mm, inside diameter 4.5mm, wall thickness 0.25mm, as drawn, 99.5%

GF50403440

Iron, tube, 1000mm, outside diameter 12.0mm, inside diameter 10mm, wall thickness 1.0mm, annealed, 99.5%

GF18645240

Iron, tube, 100mm, outside diameter 10.0mm, inside diameter 7mm, wall thickness 1.5mm, annealed, 99.5%

GF88344108

Iron, tube, 200mm, outside diameter 12.0mm, inside diameter 10mm, wall thickness 1.0mm, annealed, 99.5%

GF55566816

Iron, tube, 500mm, outside diameter 1.0mm, inside diameter 0.8mm, wall thickness 0.1mm, as drawn, 99.5%

GF31151887

Iron, tube, 200mm, outside diameter 2.0mm, inside diameter 1.6mm, wall thickness 0.2mm, as drawn, 99.5%

GF21950197

Iron, tube, 1000mm, outside diameter 3.18mm, inside diameter 1.96mm, wall thickness 0.61mm, as drawn, 99.5%

GF97078281

Iron, tube, 500mm, outside diameter 1.65mm, inside diameter 0.89mm, wall thickness 0.38mm, as drawn, 99.8+%

GF28189525

Iron, tube, 200mm, outside diameter 12.7mm, inside diameter 9.5mm, wall thickness 1.6mm, annealed, 99.5%

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Iron, tube, 200mm, outside diameter 2.38mm, inside diameter 1.6mm, wall thickness 0.39mm, as drawn, 99.8+%

GF35765826

Iron, tube, 100mm, outside diameter 3.18mm, inside diameter 1.96mm, wall thickness 0.61mm, as drawn, 99.5%

GF55674164

Iron, tube, 1000mm, outside diameter 5.0mm, inside diameter 4.5mm, wall thickness 0.25mm, as drawn, 99.5%

GF82933511

Iron, tube, 1000mm, outside diameter 3.2mm, inside diameter 2.2mm, wall thickness 0.5mm, as drawn, 99.8%

GF30656077

Iron, tube, 200mm, outside diameter 10.0mm, inside diameter 7mm, wall thickness 1.5mm, annealed, 99.5%

GF93209328

Iron, tube, 200mm, outside diameter 8.0mm, inside diameter 5mm, wall thickness 1.5mm, annealed, 99.5%

GF39321390

Iron, tube, 100mm, outside diameter 10.6mm, inside diameter 10.0mm, wall thickness 0.3mm, as drawn, 99.8+%

GF38919279

Iron, tube, 200mm, outside diameter 20.0mm, inside diameter 16mm, wall thickness 2.0mm, annealed, 99.5%

GF15174255

Iron, tube, 200mm, outside diameter 10.6mm, inside diameter 10.0mm, wall thickness 0.3mm, as drawn, 99.8+%