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ISEcp1-mediated transposition of qnrB-like gene in Escherichia coli.

Antimicrobial agents and chemotherapy (2008-06-04)
Vincent Cattoir, Patrice Nordmann, Jesus Silva-Sanchez, Paula Espinal, Laurent Poirel
ABSTRACT

A novel QnrB-like plasmid-mediated resistance determinant, QnrB19, was identified from an Escherichia coli clinical isolate from Colombia. Its gene was associated with an ISEcp1-like insertion element that did not act as a promoter for its expression. Using an in vitro model of transposition, we showed that the ISEcp1-like element was able to mobilize the qnrB19 gene.

MATERIALS
Product Number
Brand
Product Description

Supelco
Ciprofloxacin, VETRANAL®, analytical standard
Supelco
Norfloxacin, VETRANAL®, analytical standard
Supelco
Ofloxacin, VETRANAL®, analytical standard
Sigma-Aldrich
Ciprofloxacin, ≥98% (HPLC)
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Enrofloxacin, VETRANAL®, analytical standard
Sigma-Aldrich
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Nalidixic acid, ≥98%
Sigma-Aldrich
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Norfloxacin, analytical standard, ≥98% (TLC)