821841
TSKgel® ODS-100V (5 µm) HPLC Columns
L x I.D. 1 cm × 2 mm, C18 (octadecyl) phase
About This Item
product name
TSKgel® ODS-100V Guard Cartridge, phase C18 (octadecyl), L × I.D. 1 cm × 2 mm, 5 μm particle size
material
stainless steel
Agency
suitable for USP L1
product line
TSKgel®
manufacturer/tradename
Tosoh 21841
extent of labeling
15% Carbon, endcapped with bifunctional dialkylsilane
parameter
0-100% organic solvent
10-50 °C temperature
technique(s)
HPLC: suitable
L × I.D.
1 cm × 2 mm
surface area
450 m2/g
matrix
spherical silica
matrix active group
C18 (octadecyl) phase
particle size
5 μm
operating pH
2.0-7.5
separation technique
reversed phase
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General description
The bonded phase in TSKgel ODS-100V columns is prepared by an incomplete first reaction with a difunctional octadecylsilane reagent, which is followed by endcapping with a mixture of two difunctional dialkylsilane reagents. Both reactions are performed under anhydrous conditions to promote the formation of a monomeric C18 bonded phase structure. The carbon content of TSKgel ODS-100V is 15%, while the carbon content of the fully bonded TSKgel ODS-100Z columns is 20%. Thus, while non-polar, the polarity of the ODS-100V stationary phase is high, which favors the retention of polar organic compounds. Thus, TSKgel ODS-100V columns can be used with mobile phases that do not contain organic solvent, making this column type very suitable for the analysis of very hydrophilic compounds.
Reversed-phase liquid chromatography (RPLC) is the preferred method for analysis and purification of polar and nonpolar compounds that are soluble in mixtures of water and organic solvents.
Physical form
Legal Information
accessory
analytical column
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