ZRB1210

Anti-CNKSR1 Antibody, clone 4B7 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Connector enhancer of kinase suppressor of ras 1;Connector enhancer of KSR 1;CNK homolog protein 1;CNK1;hCNK1;Connector enhancer of KSR-like
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4B7, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 79.71 and 78.79 kDa; observed mol wt ~81 and 79 kDa
• purified by: using Protein A
• species reactivity: mouse, human
• species reactivity (predicted by homology): rat
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_001284576.1
• UniProt accession no.: Q969H4
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 4B7 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Connector enhancer of kinase suppressor of ras 1 (CNKSR1). It targets an epitope within 21 amino acids from the C-terminal region. It detects both isoforms of CNKSR1.

Immunogen

KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human Connector enhancer of kinase suppressor of ras 1 (CNKSR1).

Application

Quality Control Testing

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CNKSR1 in K562 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CNKSR1 in lysates from HepG2 cells and Mouse Heart tissue.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CNKSR1 in K562 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CNKSR1 in human pancreas tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Connector enhancer of kinase suppressor of ras 1 (UniProt: Q969H4; also known as Connector enhancer of KSR 1, CNK homolog protein 1, CNK1, hCNK1, Connector enhancer of KSR-like, CNKSR1) is encoded by the CNKSR1 (also known as CNK1) gene (Gene ID: 10256) in human. CNKSR1 is a peripheral membrane protein that serves as an adapter protein and regulates Ras signaling pathways through interactions with proteins of distinct pathways in the cytoplasm. One of its main function is to regulate cancer-related signaling pathways and integrate output to different intracellular signaling cascades upon stimulation by extracellular cues. Its activity is closely regulated by phosphorylation at tyrosines 26, 519, and 665. Phosphorylation of Tyr 519 is shown to recruit CNKSR1 to the nucleus and additional phosphorylation of tyrosine 26 enables it to promote SRE-dependent gene expression. Overexpression of CNKSR1 has been reported in pancreatic cancer tissue and it is suggested to be an independent prognostic marker of overall survival. At its N-terminal it has a sterile motif (SAM; aa 7-70), followed by a conserved region in Cnk (CRIC; aa 78-164), a), a PDZ domain (aa 196-285), and a pleckstrin homology (PH) domain (aa 403-502). Two isoforms of CNKSR1 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Quadri, HS., et al. (2017). BMC Cancer 17: Article number: 495; Fischer, A., et al. (2015). Biochim. Biophys. Acta. 1853(11 Pt A); 2847-2855).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable