ligand
sulphopropyl
description
Ion Exchanger Type (value)
packaging
pkg of 20 mL
manufacturer/tradename
Cytiva 28-9365-40
parameter
1.5 bar (22 psi) (Over the Packed Bed During Operation)
2 ml/min - 10 ml/min flow rate
22 psi
bed size
16 mm × 100 mm
bed volume
20 mL
column I.D.
16 mm
matrix
6% cross-linked agarose with dextran surface extenders
particle size
45-165 μm
average diameter
90 μm
cleaning
3-14
working range
4-13
suitability
suitable for bioprocess medium
General description
Application
Features and Benefits
- Provide high loading capacity and fast purifications.
- Optimized for reliable, reproducible separations.
- Compatible with simple pump-based configurations and chromatography systems such as ÄKTA® design.
- Excellent choice for rapid enrichment during initial capture of Proteins from start material.
- Strong Sulfopropyl (SP) cation exchanger.
Storage and Stability
Analysis Note
Other Notes
Legal Information
related product
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
Protocols
This page covers how and when to use Sepharose XL media for purification of proteins.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
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