00879

8-Acetoxypyrene-1,3,6-trisulfonic acid trisodium salt

for fluorescence, ≥98.0% (TLC)

• Synonym(s): 1-Acetoxypyrene-3,6,8-trisulfonic acid trisodium salt, Trisodium-8-acetoxypyrene-1,3,6-trisulfonate
• Empirical Formula (Hill Notation): C₁₈H₉Na₃O₁₁S₃
• CAS Number: 115787-83-2
• Molecular Weight: 566.42
• Beilstein: 9538267
• MDL number: MFCD00135685
• UNSPSC Code: 12352200
• PubChem Substance ID: 24845099

Properties

• grade: for fluorescence
• Assay: ≥98.0% (TLC)
• mp: 238-248 °C (dec.) (lit.)
• solubility: DMF: soluble; DMSO: soluble; H₂O: soluble; methanol: soluble
• fluorescence: λ_ex 460 nm; λ_em 510 nm in 0.1 M potassium phosphate pH 8.0 (esterase)
• storage temp.: −20°C
• SMILES string: [Na+].[Na+].[Na+].CC(=O)Oc1cc(c2ccc3c(cc(c4ccc1c2c34)S([O-])(=O)=O)S([O-])(=O)=O)S([O-])(=O)=O

Description

Application

A membrane permeable, nonfluorescent reagent . This fluorochrome enters the cell cytoplasm, where its acetic group is cleaved by intracellular, nonspecific esterases, which forms the impermeable, fluorescent form of the molecule. Utilized as a phloem-mobile symplasmic tracer having staining characteristics that differ from the commonly used CFDA (5-(6-)carboxyfluorescein diacetate).

suitable as fluorogenic substrate for esterases

Other Notes

Highly water soluble enzyme substrate for the fluorimetric assay of esterases at longwave excitation and emission wavelengths