Abstract
Cell culture is an essential technique in modern biological laboratories and is employed in a wide range of research fields, including oncology, genetics, pharmacology, and bioproduction. Cell line contamination and misidentification is a significant threat facing cell culture, with the potential to invalidate years, if not decades, of data. Other common obstacles to research reproducibility involving culture systems include environmental variability, media inefficiency, and inappropriate scaling up or down of operations. Addressing these challenges will ensure the continued utility and reliability of cell culture across the biological sciences. Experts from Public Health England share tips from the European Collection of Authentic Cell Cultures (ECACC) labs on how to avoid and resolve common cell culture issues, while ensuring credibility and sustainability for data obtained from your cell culture models.
In this webinar, you will learn:
- Choosing the best culture setup for modeling your biological system
- Processes that will minimize variations and enhance culture reproducibility
- Why (and how) every culture room must be screened for mycoplasma contamination
- How to confirm cell line identity, and best practices that will help you avoid cross-contamination
Speakers
Sharon Bahia PhD
National Infection Service, Public Health England (PHE)
Product & Distributor Manager, Culture Collections
Jim Cooper
ECACC
Cell Biology Application Scientist
Jim Cooper is a Cell Biology Application Scientist working in the Public Health England (PHE) Culture Collections Scientific development Group. He has over 20 years’ experience of delivering authentic cell cultures for researchers in academia and industry and has a wide and deep knowledge of cell biology applications and how to integrate them with cell culture processes. For many years Jim played a key role in ECACC, managing internal development projects, custom cell culture projects and ECACC's internationally renowned cell culture training courses. Jim is a member of the International Cell Line Authentication Committee (ICLAC) and has a growing reputation as an enthusiastic speaker, delivering many seminars on good cell culture practice to Institutes and companies across the UK and Europe and carrying out site visits to helptrouble-shoot cell culture problems. Jim is currently is developing and characterising novel in vitro models.
Cell culture and analysis
- Microbial cell culture
Duration:1h 27min
Language:English
Session 1:presented June 11, 2018
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