WellRED Oligos
Designed for use with the CEQ/GeXP Genetic Analysis Systems, we are pleased to offer WellRED dye-labeled oligos under our license from Beckman Coulter, Inc. In addtion to their designed application, these labeled oligos may also be used in direct hybridization or PCR amplification. WellRED dye-labeled oligos are DNA oligonucleotides labeled with WellRED dyes, D2, D3 or D4, at the 5'end.
WellRED Specifiations | |
|---|---|
| Yields | Cartridge: 2 and 4 OD HPLC: 5, 10, 50 and 100 OD |
| Purification | Cartridge, HPLC |
| Length | 15 – 30 bases (inquire for longer lengths) |
| Backbone | Phosphodiester |
| Format | Supplied Dry |
| Modifications | D2-PA, D3-PA or D4-PA at the 5’end |
| Packaging | 2 mL Opaque Tubes |
| Quality Controls | MALDI-TOF MS, Electrospray Ionization MS, OD by UV Spectroscopy |
| Technical Datasheet & Labels | Includes yield, Tm (melting temperature), MW, and µg/OD Duplicate set of labels with each order |
| Additional Services | Aliquoting into tubes MSQC traces available upon request |
| Shipping Schedule | Shipped within 4 to 5 business days |
Benefits of WellRED Dye Chemistry
- WellRED oligos use cyanine-based fluorescent dyes with high extinction coefficients that absorb in the near-infrared region, thereby reducing background noise from biological materials, resulting in highly sensitive detection.
- The CEQ/GeXP Genetic Analysis Systems enable DNA samples to be kept in linear form during analysis. The CEQ/GeXP Genetic Analysis Systems' online denaturation process prevents any reformation of secondary structures, especially during a long run without any manual steps.
- WellRED dye chemistry and pre-heating enable the CEQ/GeXP Genetic Analysis Systems to resolve complex structures, where other systems fail.
Yields and Estimated Number of Assays | ||||
|---|---|---|---|---|
| Yield (OD) | 2 | 4 | 5 | 10 |
| Purification | Cartridge | Cartridge | HPLC | HPLC |
| Approx. yield (nmols*) | 10 | 20 | 25 | 50 |
| Estimated assays (reactions) | 1,000-2,000 | 2,000-4,000 | 2,500-5,000 | 5,000-10,000 |
*Estimate 1 OD = 5 nmols = 30 µg, for a 20 base oligo
Spectral Properties of WellRED Dyes | |||
|---|---|---|---|
| WellRED Oligo | Excitation maximum (nm) | Emission maximum (nm) | Molar extinction coefficient |
| D2-PA | 750 | 770 | 170,000 |
| D3-PA | 685 | 706 | 224,000 |
| D4-PA | 650 | 670 | 203,000 |
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